ScaI

ScaI
CT10S401-01 500 units
CT10S401-02 2x500 units
Storage
-20° C
Concentration
20,000 units/ml
Recognition Site
5’…AGT^ACT…3’
3’…TCA^TGA…5’
Description
ScaI is expressed and purified from E.coli that carries the recombinant ScaI gene. The molecular weight is 27.8 kDa, with the recognition site at AGT^ACT. The optimised digestion can be achieved in 5-15 minutes at 37℃ and terminated at 80℃ for 20 minutes by heat inactivation.This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.
SmaI
CT10S501-01 1,000 units
CT10S501-02 2×1,000 units
Storage
-20° C
Concentration
20,000 units/ml
Recognition Site
5’…CCC^GGG…3’
3’…GGG^CCC…5’
Description
SmaI is expressed and purified from E.coli that carries the recombinant SmaI gene. The molecular weight is 29.7 kDa, with the recognition site at CCC^GGG. The optimised digestion can be achieved in 5-15 minutes at 37℃ and terminated at 80℃ for 20 minutes by heat inactivation.This enzyme is not sensitive to dam, dcm, but sensitive to mammalian CpG methylation.
SpeI
CT10S601-01 250 units
CT10S601-02 2×250 units
Storage
-20° C
Concentration
20,000 units/ml
Recognition Site
5’…A^CTAGT…3’
3’…TGATC^A…5’
Description
SpeI is expressed and purified from E.coli that carries the recombinant SpeI gene. The molecular weight is 21.7 kDa, with the recognition site at A^CTAGT. The optimised digestion can be achieved in 5-15 minutes at 37℃ and terminated at 80℃ for 20 minutes by heat inactivation.This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.
SphI
CT10S701-01 250 units
CT10S701-02 2x250 units
Storage
-20° C
Concentration
20,000 units/ml
Recognition Site
5’…GCATG^C…3’
3’…C^GTACG…5’
Description
SphI is expressed and purified from E.coli that carries the recombinant SphI gene. The molecular weight is 26.8 kDa, with the recognition site at GCATG^C. The optimi sed digestion can be achieved in 5-15 minutes at 37℃ and terminated at 80℃ for 20 minutes by heat inactivation.This enzyme is not s e n s iti v e t o d a m , d c m
XbaI
CT10X101-01 1,500 units
CT10X101-02 2×1,500 units
Storage
-20° C
Concentration
20,000 units/ml
Recognition Site
5’…T^CTAGA…3’
3’…AGATC^T…5’
Description
XbaI is expressed and purified from E.coli that carries the recombinant XbaI gene. The molecular weight is 24.7 kDa, with the recognition site at T^CTAGA. The optimised digestion can be achieved in 5-15 minutes at 37℃ and terminated at 80℃ for 20 minutes by heat inactivation. This enzyme is not sensitive to dcm or mammalian CpG methylation, but sensitive to dam methylation.
XhoI
CT10X201-01 2,500 units
CT10X201-02 2×2,500 units
Storage
-20° C
Concentration
20,000 units/ml
Recognition Site
5’…C^TCGAG…3’
3’…GAGCT^C…5’
Description
XhoI is expressed and purified from E.coli that carries the recombinant XhoI gene. The molecular weight is 27.9 kDa, with the recognition site at C^TCGAG. The optimised digestion can be achieved in 5-15 minutes at 37℃ and terminated at 80℃ for 20 minutes by heat inactivation. This enzyme is not sensitive to dam or dcm methylation, but sensitive to mammalian CpG methylation.
XmaI
CT10X301-01 250 units
CT10X301-02 2×250 units
Storage
-20° C
Concentration
10,000 units/ml
Recognition Site
5’…C^CCGGG…3’
3’…GGGCC^C…5’
Description
XmaI is expressed and purified from E.coli that carries the recombinant XmaI gene. The molecular weight is 37.6 kDa, with the recognition site at C^CCGGG. The optimised digestion can be achieved in 5-15 minutes at 37℃ and terminated at 80℃ for 20 minutes by heat inactivation.This enzyme is not sensitive to dam or dcm methylation, but sensitive to mammalian CpG methylation.
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