NcoI

NcoI
CT10N101-01 500 units
CT10N101-02 2x500 units
Storage
-20° C
Concentration
20,000 units/ml
Recognition Site
5’…C^CATGG…3’
3’…GGTAC^C…5’
Description
NcoI is expressed and purified from E.coli that carries the recombinant NcoI gene. The molecular weight is 32.3 kDa, with the recognition site at C^CATGG. The optimised digestion can be achieved in 5-15 minutes at 37℃ and terminated at 80℃ for 20 minutes by heat inactivation. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.
NdeI
CT10N201-01 2,000 units
CT10N201-02 2×2,000 units
Storage
-20° C
Concentration
20,000 units/ml
Recognition Site
5’…CA^TATG…3’
3’…GTAT^AC…5’
Description
NdeI is expressed and purified from E.coli that carries the recombinant NdeI gene. The molecular weight is 46.5 kDa, with the recognition site at CA^TATG. The optimised digestion can be achieved in 5-15 minutes at 37℃ and terminated at 80℃ for 20 minutes by heat inactivation. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.
NheI
CT10N301-01 500 units
CT10N301-02 2×500 units
Storage
-20° C
Concentration
20,000 units/ml
Recognition Site
5’…G^CTAGC…3’
3’…CGATC^G…5’
Description
NheI is expressed and purified from E.coli that carries the recombinant NheI gene. The molecular weight is 39.1 kDa, with the recognition site at G^CTAGC. The optimized digestion can be achieved in 5-15 minutes at 37℃ and terminated at 80℃ for 20 minutes by heat inactivation. This enzyme is not sensitive to dam or dcm methylation, but sensitive to mammalian CpG methylation.
NotI
CT10N401-01 250 units
CT10N401-02 2x250 units
Storage
-20° C
Concentration
2 0 , 0 0 0 u n it s / m l units/ml
Recognition Site
5’…GC^GGCCGC…3’
3’…CGCCGG^CG…5’
Description
NotI is expressed and purified from E.coli that carries the recombinant NotI gene. The molecular weight is 43.3 kDa, with the recognition site at GC^GGCCGC. The optimized digestion can be achieved in 5-15 minutes at 37℃ and terminated at 80℃ for 20 minutes by heat inactivation. This enzyme is not s e n s iti v e t o d a m o r d c m methylation, but sensitive to mammalian CpG methylation.
PstI
CT10P101-01 5,000 units
CT10P101-02 2×5,000 units
Storage
-20° C
Concentration
20,000 units/ml
Recognition Site
5’…CTGCA^G…3’
3’…G^ACGTC…5’
Description
PstI is expressed and purified from E.coli that carries the recombinant PstI gene. The molecular weight is 38.3 kDa, with the recognition site at CTGCA^G. The optimised digestion can be achieved in 5-15 minutes at 37℃ and terminated at 80℃ for 20 minutes by heat inactivation.This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.
PvuI
CT10P201-01 250 units
CT10P201-02 2×250 units
Storage
-20° C
Concentration
20,000 units/ml
Recognition Site
5’…CGAT^CG…3’
3’…GC^TAGC…5’
Description
PvuI is expressed and purified from E.coli that carries the recombinant PvuI gene. The molecular weight is 30.8 kDa, with the recognition site at CGAT^CG. The optimized digestion can be achieved in 5-15 minutes at 37℃ and terminated at 80℃ for 20 minutes by heat inactivation.This enzyme is not sensitive to dam, dcm, but sensitive to mammalian CpG methylation.
SacI
CT10S101-01 1,000 units
CT10S101-02 2×1,000 units
Storage
-20° C
Concentration
20,000 units/ml
Recognition Site
5’…GAGCT^C…3’
3’…C^TCGAG…5’
Description
SacI is expressed and purified from E.coli that carries the recombinant SacI gene. The molecular weight is 40.8 kDa, with the recognition site at GAGCT^C. The optimised digestion can be achieved in 5-15 minutes at 37℃ and terminated at 80℃ for 20 minutes by heat inactivation.This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.
SacII
CT10S201-01 1,000 units
CT10S201-02 2x1,000 units
Storage
-20° C
Concentration
20,000 units/ml
Recognition Site
5’…CCGC^GG…3’
3’…GG^CGCC…5’
Description
SacII is expressed and purified from E.coli that carries the recombinant SacII gene. The molecular weight is 32.1 kDa, with the recognition site at CCGC^GG. The optimised digestion can be achieved in 5-15 minutes at 37℃ and terminated at 80℃ for 20 minutes by heat inactivation.This enzyme is not sensitive to dam, dcm, but sensitive to mammalian CpG methylation.
SalI
CT10S301-01 1,000 units
CT10S301-02 2x1,000 units
Storage
-20° C
Concentration
20,000 units/ml
Recognition Site
5’…G^TCGAC…3’
3’…CAGCT^G…5’
Description
SalI is expressed and purified from E.coli that carries the recombinant SalI gene. The molecular weight is 36.3 kDa, with the recognition site at G^TCGAC. The optimised digestion can be achieved in 5-15 minutes at 37℃ and terminated at 80℃ for 20 minutes by heat inactivation. This enzyme is not sensitive to dam, dcm, but sensitive to mammalian CpG methylation.
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