AvrII
AvrII
| CT10A101-01 | 50 units |
| CT10A101-02 | 2x50 units |
Storage
-20° C
Concentration
5,000 units/ml
Recognition Site
5’…C^CTAGG…3’
3’…GGATC^C…5′
3’…GGATC^C…5′
Description
AvrII is expressed and purified from
E.coli that carries the recombinant
AvrII gene. The molecular weight is
42.3 kDa, with the recognition site at
C^CTAGG. The reaction is optimised
for 5-15 minutes at 37℃, and heatinactivated at 80℃ for 20 minutes.
This enzyme is not sensitive to dam,
dcm or mammalian CpG methylation.
Applications Genomic DNA, plasmid
DNA, PCR product.
BamHI
| CT10B101-01 | 5,000 units |
| CT10B101-02 | 2×5,000 units |
Storage
-20° C
Concentration
20,000 units/ml
Recognition Site
5’…G^GATCC…3’
3’…CCTAG ^G…5’
3’…CCTAG ^G…5’
Description
BamHI is expressed and purified from
E.coli that carries the recombinant
BamHI gene. The molecular weight is
27.5 kDa, with the recognition site at
G^GATCC. The optimised digestion
can be achieved in 5-15 minutes at
37℃ and terminated at 80℃ for 20
minutes by heat inactivation.This
enzyme is not sensitive to dam, dcm
or mammalian CpG methylation.
BglII
| CT10B201-01 | 1,000 units |
| CT10B201-02 | 2×1,000 units |
Storage
-20° C
Concentration
10,000 units/ml
Recognition Site
5’…A ^GATCT…3’
3’…TCTAG ^A…5
3’…TCTAG ^A…5
Description
BglII is expressed and purified from
E.coli that carries the recombinant
BglII gene. The molecular weight is
26.7 kDa, with the recognition site at
A^GATCT. The reaction is optimized
for 5-15 minutes at 37°C, and heatinactivated at 80℃ for 20 minutes.
This enzyme is not sensitive to dam,
dcm or mammalian CpG methylation.
BsgI
| CT10B301-01 | 50 units |
| CT10B301-02 | 2x50 units |
Storage
-20° C
Concentration
5,000 units/ml
Recognition Site
5’…GTGCAG(N) 16 ^…3’
3’…CACGTC(N) 14 ^ …5’
3’…CACGTC(N) 14 ^ …5’
Description
BsgI is expressed and purified from
E.coli that carries the recombinant
BsgI gene. The MW is 122 kDa, with
t h e r e c o g n i t i o n s i t e a t
Ë„ GTGCAG(N)14 (N)â‚‚ . The optimized Ë…
digestion can be achieved in 5-15
minutes at 37℃ and terminated at
80℃ for 20 minutes by heat
inactivation.This enzyme is not
s e n s iti v e t o d a m , d c m o r
mammalian CpG methylation.
EagI
| CT10E101-01 | 250 units |
| CT10E101-02 | 2×250 units |
Storage
-20° C
Concentration
20,000 units/ml
Recognition Site
5’…C^GGCCG…3’
3’…GCCGG^C…5’
3’…GCCGG^C…5’
Description
EagI is expressed and purified from
E.coli that carries the recombinant
EagI gene. The molecular weight is
35.1 kDa, with the recognition site at
C^GGCCG. The optimised digestion
can be achieved in 5-15 minutes at
37℃ and terminated at 80℃ for 20
minutes by heat inactivation. This
enzyme is not sensitive to dam or dcm
methylation, but sensitive to
mammalian CpG methylation.
EcoRI
| CT10E201-01 | 5,000 units |
| CT10E201-02 | 2×5,000 units |
Storage
-20° C
Concentration
20,000 units/ml
Recognition Site
5’…G^AATTC…3’
3’…CTTAA^G…5’
3’…CTTAA^G…5’
Description
EcoRI is expressed and purified from
E.coli that carries the recombinant
EcoRI gene. The molecular weight is
32.0 kDa, with the recognition site at
G^AATTC. The optimised digestion
can be achieved in 5-15 minutes at
37℃ and terminated at 80℃ for 20
minutes by heat inactivation.This
enzyme is not sensitive to dam, dcm
methylation, but sensitive to
mammalian CpG methylation.
EcoRV
| CT10E301-01 | 2,000 units |
| CT10E301-02 | 2x2,000 units |
Storage
-20° C
Concentration
20,000 units/ml
Recognition Site
5’…GAT^ATC…3’
3’…CTAT^AG…5’
3’…CTAT^AG…5’
Description
EcoRV is expressed and purified from
E.coli that carries the recombinant
EcoRV gene. The molecular weight is
29.5 kDa, with the recognition site at
GAT^ATC. The optimised digestion
can be achieved in 5-15 minutes at
37℃ and terminated at 80℃ for 20
minutes by heat inactivation.This
enzyme is not sensitive to dam or dcm
methylation, but sensitive to
mammalian CpG methylation.
HindIII
| CT10H101-01 | 5,000 units |
| CT10H101-02 | 2x5,000 units |
Storage
-20° C
Concentration
20,000 units/ml
Recognition Site
5’…A^AGCTT…3’
3’…TTCGA^A…5’
3’…TTCGA^A…5’
Description
HindIII is expressed and purified from
E.coli that carries the recombinant
HindIII gene. The molecular weight is
35.8 kDa, with the recognition site at
A^AGCTT. The optimized digestion
can be achieved in 5-15 minutes at
37℃ and terminated at 80℃ for 20
minutes by heat inactivation.This
enzyme is not sensitive to dam, dcm
or mammalian CpG methylation.
KpnI
| CT10K101-01 | 2,000 units |
| CT10K101-02 | 2x2,000 units |
Storage
-20° C
Concentration
10,000 units/ml
Recognition Site
5’…GGTAC^C…3’
3’…C^CATGG…5’
3’…C^CATGG…5’
Description
KpnI is expressed and purified from
E.coli that carries the recombinant
KpnI gene. The molecular weight is
25.9 kDa, with the recognition site at
GGTAC^C. The optimised digestion
can be achieved in 5-15 minutes at
37℃ and terminated at 80℃ for 20
minutes by heat inactivation.This
enzyme is not sensitive to dam, dcm
or mammalian CpG methylation.c
DNA, plasmid DNA, PCR product.