T5 Exonuclease

T5 Exonuclease
CT12X101-01 50 units
CT12X101-02 5x50 units
Concentration
10000 units/ml
Contents
  • T5 Exonuclease
  • 10×T5 Exonuclease Reaction Buffer
  • 1 0 × D N A L o a d i n g Buffer
Storage
-20° C
Description
This product is produced by inducing expression and purification of recombinant T5 phage D15 gene in Escherichia coli, with a molecular weight of about 33.2 kDa. T5 Exonuclease degrades DNA along the 5´→3´ direction. It can not only digest from the 5´ end, but also from the cut or gap of linear or circular double-stranded DNA, but it cannot degrade supercoiled double-stranded DNA. T5 Exonuclease also has single-stranded DNAendonuclease activity.
Unit Definition
In a 50 μl reaction system, the amount of enzyme required to catalyze the production of 1 nmol of acid-soluble deoxyribonucleotides from a 0.15 mM double-stranded DNA substrate within 30 minutes at 37°C is defined as 1 activity unit (U).
Applications
  • In vitro gene site-directed mutations degrade the methylated plasmid template.
Uracil-DNA Glycosylase
CT12U101-01 1000 units
CT12U10102 5 x 1000 units
Concentration
5000 units/ml
Contents
  • UDG Enzyme
  • 10×UDG Reaction Buffer
  • 1 0 × D N A L o a d i n g Buffer
Storage
-20° C
Description
This product is a 26.5 kDa purified recombinant protein inducibly expressed in E. coli carrying the Uracil-DNAGlycosylase gene. UDG enzyme catalyzes the release of uracil in single-stranded or double-stranded DNA but is not effective for oligomeric DNA(n≤6 bases)
Highlights
Remove uracil from DNA.
Unit Definition
One unit is defined as the amount of enzyme required to release 60 pM uracil from uracil-containing double-stranded DNA per minute at 37℃ in a 50 μl reaction system.
Applications
  • Prevent residual DNA contamination and improve the specificity of PCR products.
dsDNase
CT12D101-01 50 units
CT12D101-02 5 x 50 units
Concentration
3000 units/ml
Contents
  • dsDNase
  • 10×dsDNase Reaction Buffer
  • RNase-free Water
Storage
-20° C
Description
This product is produced by induced expression and purification of recombinant dsDNase gene in E. coli, with a molecular weight of approximately 42 kDa. dsDNase is a double-strand specific endonuclease that can digest phosphodiester bonds in double-stranded DNA molecules to produce oligonucleotides with 5’ phosphate groups and 3’ hydroxyl groups. It does not digest RNA and single-stranded DNA, and is irreversibly inactivated when heated at 55°C.
Unit Definition
Using macromolecular DNA as a template, at 25°C and pH 5.0, 1 U dsDNase can increase the OD value of 260 nm absorbed light by 0.001 per minute.
Applications
  • Quickly remove the genomic DNAin RNA, which can be used for RT-PCR. In RTqPCR, gDNAis removed from RNAbefore cDNAone-strand synthesis
T7 High Efficiency Transcription Kit
CT10T101-01 20 μl×25 rxns
Storage
-20° C
Description
T7 High Efficiency Transcription Kit is designed for in vitro RNAsynthesis by T7 RNA Polymerase with supercoiled or linearized DNA templates. Up to 50 μg of RNA can be produced from a reaction of 20μL within 2 hours for 1μg template. Synthesized RNA can be used for in vitro translation, RNase protection assays, RNA splicing, and hybridization assays.
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