T4 DNA Ligase

T4 DNA Ligase
CT6L101-01 10,000 units
CT6L101-02 20,000 units
Concentration
200 units/μl
Contents
  • T4 DNALigase
  • 5×T4 DNA Ligase Buffe
Storage
-20° C
Description
T4 DNA Ligase is an ATP-dependent ligase which catalyzes the formation of a phosphodiester bond between juxtaposed 5’-phosphate and 3’-hydroxyl termini in duplex DNA or RNA . The enzyme joins blunt and cohesive ends and repairs single-strand nicks in duplex DNA, RNA or DNA/RNA hybrids but has no activity on single-strand nucleic acids.
Source
E.coli strain carrying T4 DNAligase gene
Unit Definition
One unit is the amount of enzyme required to give 50% ligation of Hind III fragments of λDNA (5’-DNA termini concentration of 0.12 μM, 200 μg/ml) in a total reaction volume of 20 μl in 30 minutes at 16°C in 1×T4 DNALigase Buffer.
Applications
  • Cloning blunt end or cohesive end fragments.
  • Ligation of synthetic linkers or adaptors
T4 DNA Ligase (for NGS)
CT12L101-01 200 Weiss U
CT12L101-02 5 x 200 Weiss U
Concentration
200 units/μl
Contents
  • T4 DNA Ligase (for NGS)
  • 10×T4 DNA Ligase Buffer
  • 50% PEG Buffer
Storage
-20° C
Description
This product is produced by inducing and purifying recombinant T4 DNA Ligase gene in E. coli, with a molecular weight of approximately 56 kDa. It can specifically catalyze the formation of phosphodiester bonds between the 5’phosphate end and the 3’hydroxyl end of double-stranded DNA or doublestranded RNA. This product has high ligation activity for sticky ends and flat ends of double-stranded nucleic acid fragments, and it also has a repair effect on nicks in double-stranded DNA, double-stranded RNAor DNA-RNAhybrid strands.
Unit Definition
In a 20μl ligation reaction system, at a 5’-end concentration of 0.12 μM (200 μg/ml), react at 16°C for 30 minutes to ligate 50% of the λDNA fragment digested by HindIII. The amount of enzyme required to ligate is defined as a viscosity Terminal activity unit (CEU). One Weiss Unit is equal to 200 CEU, and the activity unit of this product is calculated as Weiss Unit.
Applications
  • Linker connection during NGS library construction.
  • Cloning of restriction fragments.
Taq DNA Ligase
CT12L201-01 1000 units
CT12L201-02 4x1000 units
Concentration
40000 units/ml
Contents
  • T4 DNALigase
  • 10×Taq DNA Ligase Buffer
  • 1 0 × D N A L o a d i n g Buffer
Storage
-20° C
Description
This product is derived from recombinant Taq DNA Ligase gene after inducing expression and purification in E. coli, with a molecular weight of about 73 kDa. Taq DNA Ligase uses NAD+ as a cofactor to catalyze the formation of phosphodiester bonds in double-stranded DNA containing adjacent 5’-phosphate ends and 3’-hydroxyl ends. The ligation reaction can only occur when the two oligonucleotide strands are completely paired with the complementary target DNA and there is no gap between the two oligonucleotide strands. Therefore, it can be used to detect single base substitutions. In the range of 37-75℃, Taq DNA Ligase is active.
Unit Definition
In a 50 μl reaction system, at 45°C, the amount of enzyme required to connect 50% of 1 μg of λDNA fragments (12 bp sticky ends) digested by BstEII within 15 minutes is defined as 1 activity unit.
Applications
  • Incorporation of phosphorylated oligonucleotides during PCR and ligase chain reaction.
  • Specific detection of alleles by ligase detection reaction and ligase chain reaction.
  • Definition of mutagenic activity unit (sticky end activity unit) by PCR amplification and incorporation of phosphorylated oligonucleotides.
E.coli Poly (A) Polymerase
CT12A101-01 100 units
CT12A101-02 4x100 units
Concentration
5000 units/ml
Contents
  • E . c o l i P o l y ( A ) Polymerase
  • 10×E.coli Poly (A) Polymerase Buffer
  • 1 0 × D N A L o a d i n g Buffer
Storage
-20° C
Description
This product is purified from E. coli after inducible expression of a recombinant vector containing Poly (A) Polymerase gene. It has a molecular weight of 50.2 kDa. The reaction of E. coli Poly (A) Polymerase is template-independent. It catalyzes the addition of AMP converted from ATP to the 3’end of the RNA, that is, adding a poly(A) tail to the RNA.
Highlights
Template independent addition of a poly(A) tail to the 3’end of the RNA.
Unit Definition
One unit is defined as the amount of enzyme required to incorporate 1 nmol of AMP into RNAin a 50 μl reaction system in 10 minutes at 37°C.
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