Ribonuclease Inhibitor

Ribonuclease Inhibitor
CT1L101-01 2000 units
CT1L101-02 5x2000 units
Concentration
50 units/μl
Storage
-20° C
Description
Ribonuclease Inhibitor is a recombinant protein purified from E. coli strain carrying human placenta ribonuclease inhibitor gene. Ribonuclease Inhibitor specifically inhibits RNase A, RNase B, and RNase C. does not inhibit RNase 1, RNase T1, S1 nuclease, RNase H and aspergillus-originated RNase. or DNAPolymerase, AMV, M-MLV, SP6, T7 or T3 RNAPolymerases.
Unit Definition
One unit is defined as the amount of enzyme required to inhibit 5 ng Rnase Aby 50%.
Proteinase K
CT7E201-01 1 ml
Concentration
20 mg/ml
Storage
-20° C
Description
Proteinase K is a nonspecific serine protease that will hydrolyze a variety of peptide bands. Proteinase K is active in a broad range of temperature and buffers. It cannot be inactivated by metal ions, chelating agents (e.g., EDTA), or detergents such as SDS.
  • Active in a wide range of buffers and pH value.
  • Incubation temperature: 55-65℃; optimal temperature: 58℃.
  • Incubation time: 15 minutes to 48 hours; optimal incubation time: 2 hours.
Source
Purified from Tritirachium album.
Applications
  • Preparation of DNAand RNA.
  • Inactivation of RNase, DNase and enzymes.
  • Isolation of genomic DNA.
  • Isolation of RNA.
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