Phi29 DNA Polymerase

Phi29 DNA Polymerase
CT12P101-01 250 units
CT12P101-02 5x250 units
Concentration
10000 units/ml
Contents
  • P h i 2 9 D N A Polymerase
  • 1 0 × P h i 2 9 D N A Polymerase Buffer
  • P h i 2 9 R a n d o m Primers
  • 10 mM dNTPs
  • 1 0 × D N A L o a d i n g Buffer
Storage
-20° C
Description
This product is a 74.4 kDa purified recombinant protein inducibly expressed in E. coli carrying the ф29 DNA polymerase gene. With high sensitivity and great synthesis ability, it can be used for whole genome amplification from a single cell. It possesses 3’→5’ proofreading exonuclease activity to ensure high fidelity of resulting DNA. Its properties of multiple strand displacement and processive synthesis allow >10 kb lengths of amplification products from genome or plasmid DNA, and enable rolling-circle replication of circular DNA.
Highlights
  • Isothermal amplification;
  • High fidelity; high efficiency; high sensitivity; high yield;
  • Random primers or specific N9 primers can be used.
In a 50 μl reaction system, at 45°C, the amount of enzyme required to connect 50% of 1 μg of λDNA fragments (12 bp sticky ends) digested by BstEII within 15 minutes is defined as 1 activity unit.
Unit Definition
One unit is defined as the amount of enzyme required to incorporate 0.5 pmol of dNTP into a polynucleotide substrate in 10 minutes at 30℃.
Applications
  • Sequencing, SNP genotyping, STR/ microsatellite analysis.
  • Virus detection, miRNAdetection, etc.
T4 DNA Polymerase
CT12P201-01 150 units
CT12P201-02 5 x 150 units
Concentration
3000 units/ml
Contents
  • T4 DNAPolymerase
  • 2.5 mM dNTPs
  • 5×T4 DNA Polymerase Buffer
Storage
-20° C
Description
This product is produced by inducing expression and purification of recombinant T4 DNA Polymerase gene in E. coli, with a molecular weight of approximately 104 kDa. In the presence of template and primers, T4 DNA Polymerase catalyzes the synthesis of DNA along the 5’→3’ direction. This enzyme also has 3’→5’ exonuclease activity, which is stronger than DNA polymerase I. T4 DNA Polymerase does not have 5’→3’ exonuclease activity.
Unit Definition
One unit is defined as the amount of enzyme required to incorporate 10 pmol of dNTP into a polynucleotide substrate in 10 minutes at 37℃.
Applications
  • Smoothing of 5’or 3’protruding ends of DNA
  • Synthesis of labeled DNAprobes by displacement reaction
  • Subcloning after single-strand deletion
  • Synthesis of the second strand in the process of gene-directed mutagenesis.
T4 Polynucleotide Kinase
CT12K101-01 500 units
CT12K101-02 5 x 500 units
Concentration
10000 units/ml
Contents
  • T4 Polynucleotide Kinase
  • 10×T4 PNK Reaction Buffer
  • 10mM ATP
  • 1 0 × D N A L o a d i n g Buffer
Storage
-20° C
Unit Definition
Within 30 minutes at 37°C, the amount of enzyme required to transfer 1 nmol of the γ-phosphate group from ATP to the 5’-OH end of DNA is defined as 1 activity unit (U).
Applications
  • Oligonucleotide, DNA or RNA 5’end label, used as Southern, Northern, EMSA, etc. probes, DNAsequencing primers, PCR primers, etc.;
  • Phosphorylation at the 5´ end of oligonucleotides, DNAor RNA;
  • Removal of the 3´ terminal phosphate group.
T7 Endonuclease I
CT12E101-01 250 units
CT12E101-02 5 x 250 units
Concentration
10000 units/ml
Contents
  • T7 Endonuclease I
  • 10×T7 Endonuclease I Buffer
  • T7 Endonuclease I Control Template (40 ng/μl)
  • 1 0 × D N A L o a d i n g Buffer
Storage
-20° C
Highlights
High efficiency, high specificity.
Unit Definition
One unit is defined as the amount of enzyme required to convert > 90% of 1 μg of supercoiled cruciform pUC(AT) to > 90% linear form in a total reaction volume of 50 μl in 1 hour at 37°C.
Applications
  • Gene mutation and SNP detection for result from TALEN and CRISPR/CAS9
  • Recognition and cleavage for non-perfectly matched DNA and Holliday junctions
  • Randomly cleave single-stranded DNA.
Scroll to Top