DMT Enzyme (DpnI)

DMT Enzyme (DpnI)
CT7D111-01 200 units
CT7D111-02 5x200 units
Concentration
10 units/μl
Contents
  • DMT Enzyme
  • 10X Buffer
  • 6 X D N A L o a d i n g Buffer
Storage
-20° C
Description
This product is an improved version of DpnI restriction enzyme, exhibiting higher activity compared with conventional DpnI enzyme. This enzyme can effectively recognize and cleave the sequence GmATC (A is methylated) and cannot cleave the sequence GATC (A is not methylated). DMT Enzyme is compatible with reaction buffers of multiple PCR enzymes (such as QuikPfu, MetaPrime, SpeedPfu, etc.). After PCR, this enzyme can be added directly to the reaction system to start digestion reaction. After digestion reaction, downstream transformation experiments can be performed without heat inactivation.
Unit Definition
One unit is the amount of enzyme required to completely digest 1 μg of pBR322 DNA (prepared from dam+ strain) in 50 μl of reaction mixture in 1 hour at 37°C.
Applications
  • In vitro site-directed mutagenesis, digestion of methylated DNA.
Dnase I (RNase-free )
CT7D201-01 1500 units
Concentration
3 units/μl
Contents
  • 1 0 × D N a s e I Reaction Buffer
  • 200 mM EDTA DNase I (3 units/μl)
Storage
-20° C
Molecular Weight
32 kDa (monomer)
Purity
F r e e o f o t h e r D N A e n d o n u c l e a s e s a n d exonucleases, free of RNase.
Description
Deoxyribonuclease I (DNase I) is an endonuclease that digests single- and doublestranded DNA and chromatin (reaction rate is restricted by DNA association with histones). It functions by hydrolyzing phosphodiester bonds, producing mono and oligodeoxyribonucleotides with a 5’-phosphate and a 3’-hydroxyl group. Its activity depends on Ca² and it is activated by Mg² or Mn². DNase I with Mg² cleaves randomly double-stranded DNAat any site. DNase I with Mn² cleaves two DNA strands at approximately the same site to form sticky ends with 1-2 nucleotide overhangs or form blunt ends.
Unit Definition
One unit is the amount of enzyme required to completely degrade 1 μg pBR322 plasmid DNA in 10 minutes at 37°C.
Applications
  • Preparation of DNA-free RNA samples.
  • Removal of genomic DNA or other possible DNA contamination in RNA samplesl before RT-PCR.
  • Removal of DNA templates after in vitro RNA transcription catalyzed by RNAl Polymerases such as T7, T3, SP6, etc.
  • Study of DNA-protein Interactions by DNase I footprinting.
  • Nick translation.
  • Generation of a library containing random fragments of DNA
  • Generation of partially cleaved genomic DNA as a positive control in celll apoptotic TUNEL assay.
RNase A
CT7E101-01 1ml
Concentration
20 mg/ml
Storage
-20° C
Description
RNase A is a ribonuclease that cleaves single-strand RNA. It has no DNase activity.
Source
Bovine pancreas.
Unit Definition
>60 U/mg.
Applications
  • Remove RNA from DNA samples.
  • RNase protection assay.
  • Remove RNA in the processing of preparing plasmid and genomic DNA.
  • Remove RNA from solutions in recombinant protein production.
RNase H
CT12H101-01 100 units
CT12H101-02 5 x 100 units
Concentration
5,000 units/ml
Contents
  • RNase H
  • 10×RNase H Buffer
Storage
-20° C
Precautions
Fully and gently vortex 1 0 X R N a s e H B u ff e r before use.
Description
Ribonuclease H (RNase H) specifically degrades the RNA strand in RNA-DNA hybrids. It does not hydrolyze the phosphodiester bonds within single-stranded and double-stranded DNA and RNA. This product is purified from E. coli expressing the recombinant rnhA gene on a plasmid with 17 kDa molecular weight and it can be inactivated by heating at 65°C for 20 minutes.
Definition of Activity Unit
One unit is defined as the amount of RNase H that solubilizes 1 nmol RNA in the 3 tagged poly(rA)·poly(dT) hybrid chain in 20 minutes at 37°C in total reaction volume of 50 μl.
Precautions
Fully and gently vortex 10×RNase H Buffer before use.
Applications
  • Removal of mRNA prior to synthesis of second strand cDNA.
  • Identification of RNA-DNA Hybrid.
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