CARBUnn Ni-NTA Resin
Storage
2-8° C (20% ethanol)
Contents
CARBUnn Ni-NTA Resin
| CB4P101-01 | 5 ml |
| CB4P101-02 | 25 ml |
Description
CARBUnn Ni-NTA Resin allows rapid affinity purification of His-tagged
proteins, so that the His-tagged proteins are combined to the Ni-NTA
purification medium, and the uncombined proteins are eluted. The
2+ His-tagged proteins bind to Ni cations, which are immobilized on the
Ni-NTA resin by 4 metal-chelating sites. The protein combined to the
medium is gently eluted with a certain concentration of imidazole or
low pH buffer, so that, the target proteins are recovered by gradient
elution. This product has the advantages of large adsorption capacity,
good selectivity, strong permeability, and easy for regeneration. The
resin can be used for both native and denatured protein purification.
Resin Specifications
| Resin | Cross-linked 6% agarose |
| Ligand | NTA |
| Shape | sphere |
| Pore size | 45~165 μm |
| Binding capacity | 10-20 mg protein / ml wet gel |
| Recommended flow rate | <300 cm/h |
| Highest resistance of atmospheric pressure | 0.3 Mpa |
| pH stability | 3-13 |
Storage
2-8° C (20% ethanol)
Contents
CARBUnn Ni-IDA Resin
| CB4P111-01 | 5 ml |
| CB4P111-02 | 25 ml |
Description
CARBUnn Ni-IDA Resin allows rapid affinity purification of His-tagged
proteins,So that the His-tagged proteins are combined to the Ni-NTA
purification medium, and the uncombined proteins are eluted.The Histagged proteins bind to Ni2+ cations, which are immobilized on the NiIDA resin by 3 metal-chelating sites. The protein combined to the
medium is gently eluted with a certain concentration of imidazole or
low pH buffer, so that the target proteins are recovered by gradient
elution. This product has the advantages of large adsorption capacity,
good selectivity, strong permeability,and easy for regeneration.The
resin can be used for both native and denatured protein purification.
Resin Specifications
| Resin | Cross-linked 6% agarose |
| Ligand | IDA |
| Shape | sphere |
| Pore size | 90 μm |
| Binding capacity | 20-40 mg protein / ml wet gel |
| Recommended flow rate | <300 cm/h |
| Highest resistance of atmospheric pressure | 0.3 Mpa |
| pH stability | 2~14 |
Storage
2-8° C (20% ethanol)
Contents
CARBUnn GST Resin
| CB4P201-01 | 10 ml |
Description
This product is an affinity chromatography medium with glutathione as
ligand and agarose as matrix ，CARBUnn GST Resin allows rapid affinity
purification of GST-tagged proteins. It can be eluted with reduced
glutathione under mild, non-denaturing conditions, maintaining the
antigenic activity and function of the protein during the purification
process, and it is suitable for purifying GST-tagged protein and
glutathione S-transferase or glutathione-dependent protein.
Resin Specifications
| Resin | Cross-linked 4% agarose |
| Ligand | glutathione |
| Shape | sphere |
| Pore size | 90 μm |
| Ligand density | 8 mg GSH/ml wet gel |
| Dynamic binding capacity | 10-12 mg GST fusion protein / ml wet gel (MW 42 kDa) |
| Maximum flow rate (25 C) | 450 cm/h |
| Recommended flow rate | <150 cm/h |
| Highest resistance of atmospheric pressure | 0.3 Mpa |
| pH stability | 3~10 |
| Chemical stability | Stable in commonly used water-soluble buffers, stable in 70% ethnol,30% isopropanol, 6M guanidine hydrochloride, 1M acetic acid (pH4.0) |
| Physical stability | The pH or ionic strength of the solution affects the volume change rate of the medium < 2% |