QuikScript Reverse Transcriptase

QuikScript Reverse Transcriptase [M-MLV, RNase H- ]
CT1E101-02 10,000 units
CT1E101-03 5×10,000 units
Concentration
200 units/μl
Contents
  • QuikScript RT
  • 5×QS RT Buffer
  • (375 mM KCl; 15 mM MgCl2
  • 100 mM Tris-HCl pH 8.4)
  • Anchored Oligo(dT)18 Primer
Storage
-20° C
Description
QuikScript Reverse Transcriptase is an engineered version of M-MLV reverse Transcriptase with deficient RNase H activity. The enzyme is purified to near homogeneity from E.coli containing the modified M-MLV RT gene.
  • Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNAsynthesis.
  • Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
  • cDNAup to 8 kb.
Applications
  • First-strand cDNAsynthesis
  • Multiple copy gene detection
Unit Definition
One unit of QuikScript RT incorporates 1 nmol of deoxyribonucleotide into acid precipitable material in 10 minutes at 37° C using Poly(A)/Oligo(dT) as template/primer.
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