MetaScript IV One-Step gDNA Removal

MetaScript IV One-Step gDNA Removal and cDNA Synthesis SuperMix
CT1W311-02 50 rxns x 20 μl
CT1W311-03 100 rxns x 20 μl
Storage
-20° C
Description
MetaScript IV RT is a modified M – MLV reverse Transcriptase. This product uses RNA as a template. In the same reaction system, the first strand cDNA is synthesized while removing the remaining genomic DNA in the RNA template. After cDNA synthesis, gDNA remover and reverse Transcriptase are inactivated by heating at 85°C for 5 seconds. The suggested reaction temperature is 50°C with ultra-high thermal stability.
Highlights
  • Wide range of applications: high-quality RNA and degraded RNA can be used as templates for reverse Transcription.
  • Strong anti-inhibition ability: good resistance to inhibitors remaining in the RNAtemplate.
  • Speed reaction speed: Reverse Transcription and genomic DNA removal can be completed at the same time in 10 minutes.
  • Strong synthesis ability: synthesis length up to 20 kb.
  • High thermal stability: broad range reaction temperature (42°C-65°C)
  • High sensitivity: super detection rate for trace RNA.
  • After the reaction, RT/RI and gDNA Remover are simultaneously thermally inactivated. Compared with the traditional method of pretreating RNA with DNase I, it avoids the damage to RNA caused by heat-inactivated DNase I after treatment.
Applications
  • Multiple copy and low copy gene detection. Ÿ GC-rich or complex secondary structure RNA template.
  • Degraded RNA template, RNA template with high inhibitor content.
  • cDNA library construction, primer extension, 3’ and 5’RACE.
MetaScript-Uni Cell to cDNA Synthesis SuperMix for qPCR
CT1C301-01 25 rxns
Storage
-20° C
Description
MetaScript-Uni Cell to cDNA Synthesis SuperMix for qPCR uses an unique lysis buffer to lyse cells. The resulting lysate (without purification) can be directly used as template for reverse Transcription. Unique genomic DNAremover is combined with MetaScript-Uni RT/RI Enzyme Mix to achieve simultaneous genomic DNA removal and cDNA synthesis in one tube. This kit is suitable to generate qPCR-ready cDNA directly from cells.
Applications
  • Multiple copy and low copy gene detection
MetaScript-Un--Cell
MetaScript miRNA First-Strand cDNA Synthesis SuperMix
CT1T351-01 20 rxns (20 µl per reaction)
Storage
-20° C
Description
MetaScript miRNA First-Strand cDNA Synthesis SuperMix provides all the necessary components for cDNA synthesis from miRNA template. High efficient poly(A) tail addition and first-strand cDNA synthesis are performed by MetaScript miRNA RT Enzyme Mix (containing tailing enzyme and RT enzyme) and 2×MS miRNAReaction Mix.
Applications
  • miRNA synthesis
MetaScript-miRNA
MetaScript II First-Strand cDNA Synthesis SuperMix
CT1H301-02 50 rxns (20 μl per reaction)
CT1H301-03 100 rxns (20 μl per reaction)
Storage
-20° C
Description
MetaScript II First-Strand cDNA Synthesis SuperMix provides all the necessary components for cDNA synthesis from total RNA or mRNA. The cDNA first strand is efficiently synthesized by MetaScript II RT/RI Enzyme Mix and 2×MS II Reaction Mix at 42°C-55°C, of o which the optimum temperature is 50°C.
  • Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis to ensure its synthesis length and yield.
  • The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR.
  • Anchored Oligo(dT) Primer is specifically 20 designed to bind to the first base next to the 5’ end of Poly(A) tail of mRNA, providing higher specificity and high efficiency for first-strand cDNA synthesis.
  • Random Primer (N9) or Gene Specific Primer (GSP) can be used to synthesize the first-strand cDNA.
  • cDNAup to 15 kb.
MetaScript II One-Step gDNA Removal and cDNA Synthesis SuperMix
CT1H311-02 50 rxns (20 μl per reaction)
CT1H311-03 100 rxns (20 μl per reaction)
Storage
-20° C
Description
Unique genomic DNA remover is combined with MetaScript II First- Strand cDNA Synthesis SuperMix to achieve simultaneous genomic DNA removal and cDNA synthesis at 42°C-55°C. After cDNA synthesis, gDNA remover and MetaScript II RT/RI Enzyme Mix are inactivated by heating at 85°C for 5 seconds.
  • Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination.
  • 15 minutes reaction for qPCR-ready cDNA templates; 30 minutes reaction for PCRready cDNAtemplates.
  • cDNAup to 15 kb.
Applications
  • cDNAlibrary construction, 3’ and 5’RACE
  • Multiple copy and low copy gene detection
  • GC-rich or complex secondary structure RNAtemplate
Scroll to Top