MetaScript First-Strand cDNA Synthesis

MetaScript First-Strand cDNA Synthesis SuperMix
CT1T301-02 50 rxns (20 μl per reaction)
CT1T301-03 100 rxns (20 μl per reaction)
Storage
-20° C
Description
MetaScript First-Strand cDNA Synthesis SuperMix contains enzymes, dNTP, and buffer for cDNA synthesis from total RNAor mRNA.
  • Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
  • 15 minutes reaction for qPCR-ready cDNA templates; 30 minutes reaction for PCR-ready cDNAtemplates.
  • Anchored Oligo(dT) Primer for higher yield and 18 more full length cDNA.
  • cDNAup to 12 kb.
Applications
  • Multiple copy and low copy gene detection
MetaScript One-Step gDNA Removal and cDNA Synthesis SuperMix
CT1T311-02 50 rxns (20 μl per reaction)
CT1T311-03 100 rxns (20 μl per reaction)
CT1T311-04 100 rxns (20 μl per reaction)
Storage
-20° C
Description
Unique genomic DNA remover is combined with MetaScript First-Strand cDNA Synthesis SuperMix to achieve simultaneous genomic DNA removal and cDNA synthesis. After cDNA synthesis, gDNA remover and reverse Transcriptase are inactivated by heating at 85° C for 5 seconds.
  • Simultaneous genomic DNA removal and cDNA synthes i s in one tube to minimize RNA contamination.
  • 15 minutes reaction for qPCR-ready cDNA templates; 30 minutes reaction for PCR-ready cDNAtemplates.
  • cDNAup to 12 kb.
Applications
  • Multiple copy and low copy gene detection
MetaScript Hi First-Strand cDNA Synthesis SuperMix
CT1F301-02 50 rxns (20 μl per reaction)
CT1F301-03 100 rxns (20 μl per reaction)
Storage
-20° C
Description
MetaScript Hi RT is generated by site mutations. It provides high affinity to RNA template with Speed extension rate. The cDNA is efficiently synthesized by MetaScript Hi RT/RI Enzyme Mix and 2×MS Hi Reaction Mix. The entire reverse Transcription can be completed within 5 minutes.
  • 5 minutes reverse Transcription.
  • Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNAsynthesis.
  • Anchored Oligo(dT) Primer for higher yield and 18 more full length cDNA.
  • cDNAup to 12 kb.
Applications
  • Multiple copy and low copy gene detection
MetaScript-Uni One-Step gDNA Removal and cDNA Synthesis SuperMix
CT1U311-02 50 rxns (20 μl per reaction)
CT1U311-03 100 rxns (20 μl per reaction)
Storage
-20° C
Description
MetaScript-Uni RT is an improved version of M-MLV reverse Transcriptase with broad range of reaction temperature (42° C-65° C) and higher thermostability. The suggested reaction temperature is 50 C. The SuperMix contains reagents for simultaneous genomic DNA removal and cDNA synthesis. After cDNA synthesis, gDNA remover and reverse Transcriptase are inactivated by heating at 85° C for 5 seconds.
  • Broad range reaction temperature (42° C-65° C).
  • Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNAcontamination.
  • 15 minutes reaction for qPCR-ready cDNA templates; 30 minutes reaction for PCR-ready cDNAtemplates.
  • cDNAup to 20 kb.
Applications
  • Multiple copy and low copy gene detection
  • GC-rich or complex secondary structure RNAtemplate
  • cDNAlibrary construction, primer extension, 3’ and 5’RACE
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