GOLD T7 RNA & DNA Polymerase

Features
NxGn GOLD T7 RNA Polymerase
CTC0223-1 50 U/μL 25,000 U
CTC0223-2 50 U/μL 125,000 U
Storage
-20ºC
Description
T7 RNA Polymerase catalyses the 5′→3′ RNA synthesis from the T7 promoter. It is a DNA-dependent RNApolymerase cloned from the T7 bacteriophage. It recognises the T7 promoter and terminator sequences with high specificity.
  • Synthesis of RNA for nucleic acid amplification methods or gene expression studies
GOLD T7 RNA & DNA Polymerase
CTGP9201K 50 U/μL 1,000 U
CTGP9205K 50 U/μL 5,000 U
Storage
-20ºC
Description
This enzyme is a mutant form of T7 RNA polymerase (Y639F mutant). The mutation enables T7 R&DNA Polymerase to incorporate 2′-deoxyribonucleoside triphosphates (dNTPs) into full-length “RNA” transcripts more efficiently than the corresponding wild-type T7 RNApolymerase.
This mutant enzyme uses the same T7 transcription promoters as the wild-type T7 RNApolymerase.
  • Synthesis of “RNA” transcripts of mixed rNMP/2′-dNMP composition
  • Synthesis of modified “RNA” transcripts that are resistant to RNaseA
GOLD Poly(A) Tailing Kit
CTEH104H 4 U/μL 50 Reactions
Storage
-20ºC
Description
GOLD Poly(A) uses ATP as a substrate for template-independent addition of adenosine monophosphate to the 3′-OH termini of RNA molecules. The Poly(A) Polymerase Tailing Kit provides the enzyme and other reagents for quickly and easily adding a poly(A) tail to the 3′ end of any RNA.
  • Addition of a poly(A) tail to RNAsynthesised in vitro
  • Synthesis of polyadenylated RNA for nucleic acid amplification methods or gene expression studies
  • 3′-end-labeling of RNAwith radioactive Aresidues
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