Gold Rnase
GOLD RNase A
| CT012 | 5 mg/mL | 2 mL |
Storage
-20ºC
Description
GOLD RNase A is an endoribonuclease that cleaves ssRNA at the 3′
end of pyrimidine residues, forming oligoribonucleotides having
3′-terminal pyrimidine-3′-phosphates. Pyrimidine-3′-
monophosphates are also released by RNase A cleavage of
adjacent pyrimidine nucleotides. Modified RNA containing
pyrimidine-2′-fluoro-dNMPs, such as modified RNA made by in
vitro transcription using the DuraScribe T7 Transcription Kit, is
completely resistant to cleavage by RNase A.
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Removal of RNAfrom DNApreparations
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Removal of unhybridised regions of RNA from DNA-RNA or RNA-RNAhybrids
GOLD RNase I, E. coli
| CTF901K | 10 U/μL | 1,000 U |
Storage
-20ºC
Description
GOLD RNase I degrades ssRNAto nucleoside-3′-monophosphates,
via 2′,3′ cyclic monophosphate intermediates, by cleaving
between all dinucleotide pairs. This enzyme is completely
inactivated by heating at 70°C for 15 minutes, eliminating the
requirement to remove the enzyme before many downstream
applications.
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Removal of RNAfrom DNApreparations
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RNase protection assays to detect single-basepair mismatches in RNA:RNAand RNA:DNAhybrids
GOLD RNase R
| CTOG250 | 20 U/μL | 250 U |
Storage
-20ºC
Description
GOLD RNase R is a 3′→ 5′ exoribonuclease that digests essentially
all linear RNAs but will not digest lariat or circular RNA
structures. Intron RNA can be isolated from total RNA samples by
digestion with RNase R. After digestion, only circular RNAs
remain.
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Alternative splicing studies
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Gene expression studies
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CircRNA-Seq library preparation
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Intronic screening of cDNAlibraries
Hybridase GOLD RNase H
| CTC9500 | 5 U/μL | 500 U |
Storage
-20ºC
Description
Hybridase GOLD RNase H degrades the RNA in a DNA:RNA hybrid, without
affecting DNA or unhybridised RNA. In contrast to E. coli RNase H, which is
rapidly inactivated at 55 °C, this enzyme has optimal activity above 65 °C,
and can be used up to 95 °C. This property allows it to be used at
temperatures that give the highest hybridisation stringency for specific
DNA:RNA heteroduplexes, maximising sensitivity and selectivity while
minimising background due to nonspecific hybridisation.
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High-stringency hybrid selection
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Diagnostic assays of target DNAsequences
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Transcription-based amplification methods
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High-stringency mapping of mRNAstructure