GOLD CRISP Cas9 Nuclease

GOLD CRISP Cas9 Nuclease
CTG0020-1 10 mg/mL 120 μg
CTG0020-2 200 U/μL 400 μg
Storage
-20ºC
Description
GOLD CRISPR Cas9 Nuclease is a purified, recombinant wild-type Streptococcus pyogenes Cas9 nuclease produced in E. coli, containing a C-terminal 6x His tag and a C-terminal nuclear localisation sequence (NLS) to allow efficient transport to the nucleus. It is provided at high concentration (10 mg/mL, 62 μM) to enable efficient ribonucleoprotein (RNP) delivery and compatibility with multiple delivery methods, including lipidbased transfection and electroporation.
  • CRISPR/Cas9 gene-editing experiments
  • Sequence-specific, RNA-guided cleavage of dsDNA
  • Sequence enrichment and depletion
GOLD RGEN Cpf1
POR
Storage
-20ºC
Description
GOLD RGEN Cpf1 is an RNA-guided endonuclease (RGEN), with published applications including gene editing, sensitive DNA sequence detection, and cloning and assembling large DNA inserts. Several features distinguish Cpf1 from other RGEN enzymes. Cpf1 prefers an A/T-rich protospacer adjacent motif (PAM) sequence, enabling targeting of DNA sequences with high A/T content. Cpf1 cleaves DNA in a staggered fashion, similar to restriction enzymes. It does not require a tracrRNA, so guide RNAs are short.
GOLD RGEN Cpf1 is a purified, recombinant Acidaminococcus sp. Cpf1 nuclease produced in E. coli with two C-terminal nuclear localisation sequences (NLS) and one C-terminal 6 × His tag.
Published applications include:
  • CRISPR/Cpf1 gene-editing experiments
  • Sequence-specific, RNA-guided cleavage of dsDNA
  • Sequence enrichment and depletion
  • DNAsequence detection
Ready GOLD Lysozyme Solution
CTA804M 4 × 106 U
CTA810M 10 × 106 U
Storage
-20ºC
Description
Ready GOLD Lysozyme Solution is a nonmammalian, nonavian, recombinant lysozyme preparation for the lysis of Gram-negative (e.g., E. coli) and Gram-positive (e.g., Bacillus sp.) bacteria. The specific activity of Ready-Lyse Lysozyme is 200-fold higher than that of egg-white lysozyme. Additionally, it is stable at –20 °C, eliminating the need to prepare a fresh solution for each use. The use of Ready-Lyse Lysozyme results in higher yields of protein or nucleic acids than can be obtained with standard egg-white lysozyme.
  • Lysis of Gram-negative or Gram-positive bacteria for protein purification.
  • Preparation of nucleic acids from bacteria
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