GOLD Reverse Transcriptase
NxGn GOLD T7 RNA Polymerase
| CTH0125K | 200 U/μL | 25,000 U |
Storage
-20ºC
Description
MMLV GOLD Reverse Transcriptase (MMLV HP RT) demonstrates significantly greater reverse transcriptase activity than other commercially available MMLV RT enzymes. Typically, just 100 units of MMLV HP RT are required for full-length cDNA synthesis compared to 200 units of MMLV RT enzymes from many other suppliers. The enzyme synthesises full-length cDNA from RNA templates longer than 15 kb, starting with picogram amounts of RNA.
Application
-
cDNA synthesis from total RNA or poly(A)-enriched RNA for subsequent PCR, qPCR, or RNA-Seq library preparation
GOLD RNase H– Reverse Transcriptase
| CTA2910K | 200 U/μL | 10,000 U |
| CTA2925K | 200 U/μL | 25,000 U |
Storage
-20ºC
Description
GOLD Reverse Transcriptase is highly efficient at producing fulllength cDNA from RNA templates up to 12 kb. The enzyme is genetically engineered to substantially reduce RNase H activity. This structural modification eliminates degradation of RNA molecules during first-strand cDNA synthesis and gives EpiScript Reverse Transcriptase superior performance for real-time RT-PCR analysis and other applications.
Applications
-
Efficient cDNA synthesis from picogram amounts of total RNA for subsequent PCR, qPCR, or RNA-Seq library preparation
NxGn GOLD M-MuLV Reverse Transcriptase
| CTC0222-1 | 200 U/μL | 50,000 U |
| CTC0222-2 | 200 U/μL | 250,000 U |
Storage
-20ºC
Description
NxGen GOLD M-MuLV Reverse Transcriptase is an RNA-dependent DNA polymerase that shows no measurable 3′→5′ proofreading activity. This enzyme can copy a single-stranded DNAtemplate or perform cDNA synthesis by extending a DNA primer annealed to an RNAtemplate.
Applications
-
cDNA synthesis from total RNA or poly(A)-enriched RNA for subsequent PCR, qPCR, or RNA-Seq library preparation