MetaScript Probe qRT-PCR SuperMix

MetaScript Probe One-Step qRT-PCR SuperMix
CT1Q221-01 100 rxns (20 μl per reaction)
CT1Q221-02 400 rxns (20 μl per reaction)
Storage
-20ºC
Description
MetaScript Probe One-Step qRT-PCR SuperMix combines the firststrand cDNA synthesis and qPCR in the same tube to simplify reaction setup and reduce the possibility of contamination. Only gene specific primers can be used for this kit. MetaScript Probe One-Step qRT-PCR SuperMix contains all the necessary reagents for cDNA synthesis and qPCR except probe, total RNA/mRNA template and gene specific primers.
  • 5 minutes cDNAsynthesis
  • cDNAsynthesis and qPCR are performed in a single tube using gene specific primers with total RNAor mRNAas templates.
  • Passive reference dyes are provided for different qPCR instruments.
Applications
  • Multiple copy and low copy gene detection
  • Viral RNAand trace RNAdetection
PCR condions (two-step)
MetaScript II Probe One-Step qRT-PCR SuperMix
CT1Q321-01 100 rxns (20 μl per reaction)
CT1Q321-02 400 rxns (20 μl per reaction)
Storage
-20ºC
Description
MetaScript II Probe One-Step qRT-PCR SuperMix combines the first- strand cDNAsynthesis and qPCR in the same tube to simplify reaction setup and reduce the possibility of contamination. Only gene specific primers can be used for this kit. MetaScript II Probe One-Step qRT-PCR SuperMix contains all the necessary reagents for cDNA synthesis and qPCR except probe, total RNA/mRNA template and gene specific primers.
  • High specificity, high sensitivity, high efficiency.
  • Quik and rapid, reducing contamination.
  • Compatibility with various real-time cyclers with passive reference dyes provided for different qPCR instruments.
Applications
  • Multiple copy and low copy gene detection
  • GC-rich or complex secondary structure RNAtemplate
  • Viral RNAand trace RNAdetection
Thermal cycling condions (two-step)
MetaScript II Multiplex Probe One-Step qRT-PCR SuperMix UDG
CT1Q322-01 100 rxns (20 μl per reaction)
CT1Q322-02 400 rxns (20 μl per reaction)
Storage
-20ºC
Description
MetaScript II Multiplex Probe One-Step qRT-PCR SuperMix UDG is designed for one-step qRT-PCR with high sensitivity, high synthesis efficiency and high amplification efficiency. This kit firstly synthesizes first-strand cDNA with RNA as templates using reverse gene-specific primers, and then performs qPCR with the synthesized cDNA as templates using both forward and reverse gene-specific primers and fluorescent probes to achieve one step from reverse Transcription to qPCR in a single tube. After special optimization, this kit enables consistently amplifying multiple targets in a single reaction ensuring multiplex one-step RT-qPCR. dUTP/ UDG is included in the kit to degrade dU-containing ssDNA and dsDNA, which can prevent cross contamination.
  • Simple to use with reduced contamination.
  • dUTP/ UDG used to avoid cross contamination.
  • High sensitivity and high specificity.
Applications
  • Singleplex to 4-plex RT-qPCR detection.
  • High-copy and low-copy gene detection.
  • RNA templates with high GC content or complex secondary structure.
  • Detection of RNAvirus or trace amounts of RNA
Thermal cycling condions (two-step)
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