GOLD RNA 5' Polyphosphatase

GOLD RNA 5' Polyphosphatase
CTH092H 20 U/μL 200 U
Storage
-20ºC
Description
GLOD RNA 5′ Polyphosphatase is a Mg2+-independent phosphohydrolase enzyme. It sequentially removes the γ and β phosphates from 5′- triphosphorylated RNA (such as primary RNA transcripts): However, it will not dephosphorylate monophosphorylated or 5′-capped RNA. 5′ pppN—OH 3′ → 5′ pN—OH 3′ + 2 Pi
Application
  • Conversion of 5′-triphosphorylated RNA to 5′-monophosphorylated RNA for use in 5′-RNA ligation-tagging methods using T4 RNALigase
  • Analysis of 5′-end structures of RNA
  • Preparation of substrate RNA molecules for subsequent degradation using Terminator Exonuclease
GOLD T4 Polynucleotide Kinase
CT0E03K 10 U/μL 3,000 U
Storage
-20ºC
Description
GOLD T4 Polynucleotide Kinase catalyses the transfer of the γ phosphate from ATP to the 5′ OH of ssDNA and dsDNA, RNA, and nucleoside 3′-monophosphates. The enzyme also removes the 3′ p h o s p h a t e fr o m 3 ′- p h o s p h o r y l p o l y n u c l e o ti d e s , d e o x y ri b o n u c l e o s i d e 3 ′ – m o n o p h o s p h a t e s , a n d deoxyribonucleoside 3′,5′-diphosphates to form a 3′-OH group.
Application
  • Labeling of 5′ termini of DNA and RNA for DNA sequencing, blot-hybridisation, or transcript mapping
  • Phosphorylation of oligonucleotide linkers and other DNA or RNA molecules before ligation, or for use in ligation amplification with Ampligase Thermostable DNALigase
  • Preparation of labeled DNA or RNA molecular weight markers for gel electrophoresis and chromatography
GOLD DNA End-Repair Kit
CT0G20 20 Reactions
CTH1050 50 Reactions
Storage
-20ºC
Description
The GOLD DNA End-Repair Kit is used to convert DNA with damaged or incompatible 5′-protruding and/or 3′-protruding ends to 5′-phosphorylated, blunt-end DNA for the subsequent addition of next gen DNA sequencing adapters, or for cloning. After treatment of DNA with the Kit, fast and efficient blunt-end ligation can be performed using the Fast-Link DNA Ligation Kit (Lucigen).
Application
  • Treatment of enzymatically or mechanically sheared DNA or cDNAbefore adding next gen sequencing adapters
  • Polishing of enzymatically or mechanically sheared DNA, cDNA, or PCR amplicons with A overhangs, before cloning into plasmid, cosmid, fosmid, or BAC vectors.
GOLD Carbohydrases
Storage
-20ºC
Description
GOLD Carbohydrases (glycosyl hydrolases; EC 3.2.1.-) catalyze the hydrolysis of the glycosidic bond between two or more carbohydrates or between a carbohydrate and a noncarbohydrate moiety. These proteins are an increasingly important class of enzymes with numerous research and industrial applications.
Application
  • Pure: no interfering salts, sugars or colored components, Highly-concentrated enzyme solutions.
  • Recombinant: host has no endogenous biomass-degrading activities, Recombinant enzymes >90% pure.
  • Ready-to-use: no ammonium sulfate to remove before use, Extensively analyzed enzyme activity.
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