2×MetaTaq PCR SuperMix

2×MetaTaq-T PCR SuperMix
Mix (-dye) CT1S122-01 1 ml
CT1S122-02 5x1 ml
Mix (+dye) CT1S122-11 1 ml
CT1S122-12 5x1 ml
Contents
  • 2×MetaTaq-T PCR SuperMix
  • Nuclease-free Water
Storage
-20° C
Description
MetaTaq-T PCR SuperMix is a ready-to-use mixture of MetaTaq-T DNA Polymerase, dNTPs and optimized buffer. The SuperMix is provided at 2× concentration and used at 1× concentration by adding template, primers and H O. Efficiency of PCR products with “A” is 2 equal to QuikTaq DNA polymerase. It is suitable for high fidelity TAcloning.
  • Fidelity is 18-fold higher than QuikTaq DNA Polymerase.
  • Extension rate is about 1-2 kb/min
  • Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be cloned into pQUIK-T vectors.
  • Amplification of genomic DNAfragment up to 8 kb.
Applications
  • Complex templates
  • TAcloning
Thermal cycling condions
2×MetaTaq High Fidelity (HiFi) PCR SuperMix
Mix I (-dye) CT1S131-01 1 ml
CT1S131-02 5x1 ml
Mix II (-dye) CT1S131-21 1 ml
CT1S131-22 5x1 ml
Contents
  • 2×MetaTaq High Fidelity (HiFi) PCR SuperMix
  • Nuclease-free Water
Storage
-20° C
Description
MetaTaq High Fidelity (HiFi) PCR SuperMix is a readyto-use mixture of MetaTaq High Fidelity (HiFi) DNA polymerase, dNTPs and optimized buffer. MetaTaq High Fidelity (HiFi) PCR SuperMix I is optimized for the amplification of genomic DNA and PCR SuperMix II is optimized for the amplification of λDNA, cDNAor plasmid DNA. The SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers and H 2O.
  • Fidelity is 18-fold higher than QuikTaq DNA Polymerase.
  • Extension rate is about 1-2 kb/min.
  • Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be directly cloned into pQUIK-T vectors
  • Amplification of genomic DNA fragment up to 15 kb.
Applications
  • Complex templates
  • GC/AT-rich templates
  • Long PCR
  • High yield PCR
Thermal cycling condions
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