QuikTaq DNAPolymerase

QuikTaq DNA Polymerase
dNTPs-free CT1P111-01 500 units
CT1P111-02 6x500 units
CT1P111-03 4×2,500 units
CT1P111-04 10×5,000 units
dNTPs-(2.5 mM) CT1P111-11 500 units
CT1P111-12 6x500 units
CT1P111-13 4×2,500 units
Concentration
5 units/μl
Contents
  • QuikTaq DNAPolymerase
  • 10×QuikTaq Buffer (200 mM Tris-HCl pH 8.3; 200 mM Kcl; 100 mM (NH4 )2 SO4 ; 20 mM MgSO4;etc.)
  • 6×DNA Loading Buffer
Storage
-20° C
Description
QuikTaq DNA Polymerase is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. QuikTaq DNA Polymerase has 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity. It lacks 3′-5′ exonuclease activity. QuikTaq DNA Polymerase is suitable for routine amplification. PCR products are not suitable for PAGE.
  • Extension rate is about 1-2 kb/min.
  • Template-independent “A” can be generated at the 3' end of the PCR product. PCR products can be directly cloned into pQuik-T vectors.
  • Amplification of genomic DNAfragment up to 4 kb.
Applications
  • Routine PCR
  • Colony PCR
Unit Definition
One unit of QuikTaq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74° C.
Thermal cycling condions
QuikTaq DNAPolymerase for PAGE
dNTPs-free CT1P112-01 2,500 units
CT1P112-02 4×2,500 units
dNTPs (2.5 mM) CT1P112-11 2,500 units
CT1P112-12 4×2,500 units
Concentration
5 units/μl
Contents
  • QuikTaq DNA Polymerase for PAGE
  • 10×QuikTaq Buffer for PAGE (200 mM Tris-HCl pH 8.3; 200 mM KCl; 100 mM (NH 4 ) 2 SO 4 ; 20 mM MgSO 4 ; etc.)
  • 6×DNA Loading Buffer
Storage
at -20° C for two years
Description
QuikTaq DNA Polymerase for PAGE is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. QuikTaq DNAPolymerase for PAGE has 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity. lt lacks 3′-5′ exonuclease activity.
  • Extension rate is about 1-2 kb/min.
  • Unique buffer system compatible with PAGE.
  • Template-independent “A” can be generated at the 3' end of the PCR product. PCR products can be directly cloned into pQuik-T vectors.
  • Amplification of genomic DNAfragment up to 3 kb.
Applications
  • Short fragment PCR
Unit Definition
One unit of QuikTaq DNA Polymerase for PAGE incorporates 10 nmol of deoxyribonucleotide into o acid-precipitable material in 30 minutes at 74° C.
Thermal cycling condions
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