QuikPfu DNA Polymerase

QuikPfu DNA Polymerase
dNTPs-free CT1P211-01 250 units
CT1P211-02 500 units
CT1P211-03 6x500 units
dNTPs(2.5 mM) CT1P211-11 250 units
CT1P211-12 500 units
CT1P211-13 6x500 units
Concentration
2.5 units/μμl
Contents
  • QuikPfu DNAPolymerase
  • 10×QuikPfu Buffer (200 mM Tris-HCl pH 8.8; 100 mM (NH4)2 SO4; ; 100 mM KCl; 20 mM MgSO4; etc.)50 nM MgSO4
  • 6×DNA Loading Buffer
Storage
-20° C
Description
QuikPfu DNA Polymerase is an engineered version of pfu DNA Polymerase with enhanced yield and higher fidelity. QuikPfu DNA Polymerase possesses a proofreading 3′-5′ exonuclease activity
  • Fidelity is 18-fold higher than QuikTaq DNA Polymerase.
  • Extension rate is about 0.5 kb/min.
  • PCR products can be directly cloned into pQuikBlunt vectors.
  • Amplification of genomic DNAfragment up to 6 kb.
  • Amplification of plasmid DNAfragment up to 10 kb.
Applications
  • High fidelity PCR
  • Blunt end cloning
  • Site-directed mutagenesis
Unit Definition
One unit of QuikPfu DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74° C.
Thermal cycling condions
Scroll to Top