MetaSpeed Taq DNAPolymerase
MetaSpeed Taq DNAPolymerase
| dNTPs-free | CT1P101-01 | 500 units |
| CT1P101-02 | 6x500 units | |
| dNTPs(2.5 mM) | CT1P101-11 | 500 units |
| CT1P101-12 | 6x500 units |
Concentration
5 units/μl
Contents
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MetaSpeedTaq DNAPolymerase
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10×MetaSpeedTaq Buffer(200mM Tris-HCl pH 8.4; 100 mM KCl;100 mM (NH4 )2 SO4 ; 20 mM MgSO4;etc.)
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6×DNA Loading Buffer
Storage
-20º C
Description
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MetaSpeed Taq DNA Polymerase is an engineered version of Taq DNA Polymerase. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. MetaSpeed Taq DNA Polymerase has 5’-3’ DNA polymerase activity and 5’-3’ exonuclease activity. It lacks 3’-5’ exonuclease activity.
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Extension rate is about 6 kb/min.
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Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be directly cloned into pQuik-T vectors.
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Amplification of genomic DNA fragment up to 4kb.
Applications
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Routine PCR
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Speed PCR
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Colony PCR
Unit Definition
One unit of MetaSpeed Taq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74° C.
Thermal cycling condions
