NxGn GOLD T4 DNA Ligase

NxGn GOLD T4 DNA Ligase
Low Concentration CTC0241-1 2 U/μL 1,500 U
CTC0241-2 2 U/μL 75,000 U
High Concentration CTC0243-1 2 U/μL 1,500 U
CTC0243-2 2 U/μL 75,000 U
Storage
-20ºC
Description
NxGn GOLD T4 DNA Ligase is a ATP-dependent ligase commonly used for DNA cloning. It covalently joins dsDNA molecules having 5′-phosphorylated and 3′-hydroxylated blunt or compatible cohesive ends produced by restriction enzyme digestion or other enzymatic processes. It has no activity on single-stranded nucleic acids.
Application
  • Ligation of blunt or cohesive-ended DNAfragment
  • Repair of nicks in double-stranded nucleic acids
GOLD DNA Ligation Kit
CTOG05H 50 Ligations
CTF201H 100 Ligations
Storage
-20ºC
Description
Fast-Link T4 DNA Ligase provides extremely rapid, high-efficiency DNA ligation. Cohesive-end ligations can be performed in 5 minutes at room temperature. In contrast to other ligases, it is not necessary to desalt FastLink ligation reactions prior to transformation of electrocompetent or chemically competent cells.
Application
  • Blunt-end and TAcloning of PCR products
  • Ligation of next gen sequencing adapters to blunt-end DNA
  • Genomic and cDNAcloning and subcloning
  • BAC/fosmid library construction
  • Linker ligation
GOLD DNA Ligase
DNA Ligase Kit CTH101 5 U/μL 1,000 U
Enzyme and Buffer CTC2750 5 U/μL 750 U
CTC202K 5 U/μL 2,500 U
CT0A02K 100 U/μL 2,500 U
DNA Ligase CTC210K 5 U/μL 10,000 U
CT0A10K 100 U/μL 10,000 U
10X Reaction Buffer ACTA905B 10X 5 ml
Storage
-20ºC
Description
Derived from a thermophilic bacterium, GOLD DNA Ligase is stable and active at much higher temperatures than conventional DNA ligases. This enzyme catalyses NAD-dependent ligation of adjacent 3′-hydroxylated and 5′-phosphorylated termini in duplex DNA structures that are stable at high temperatures. Its half-life is 48 hours at 65 °C and >1 hour at 95 °C. It is active for at least 500 thermal cycles (94 °C/80 °C) or 16 hours of cycling, which permits extremely high hybridisation stringency and ligation specificity. It has no detectable activity in ligating blunt-ended DNA, RNA, or RNA:DNAhybrids.
Application
  • Sensitive detection of single-nucleotide polymorphisms (SNPs)
  • Ligation amplification (ligase chain reaction, LCR)
  • Gibson Assembly cloning
  • Repeat expansion detection (RED)
  • Simultaneous mutagenesis of multiple sites
GOLD T4 RNA Ligase 2
CTBD1132K 200 U/μL 2,000 U
CTBD11310K 200 U/μL 10,000 U
Storage
-20ºC
Description
GOLD T4 RNA Ligase 2, T4Rnl2(1-249), ligates single-stranded, adenylated DNA or RNA (App-DNA or App-RNA) oligonucleotides to small RNAs. The preadenylated 5′ ends of DNA or RNA are ligated to the 3′ ends of RNAin the absence of ATP, which prevents circularisation and other undesirable bimolecular reactions.
Application
  • Preparation of cDNA libraries for small-RNA transcriptome analysis, such as RNA-Seq
  • Optimal linker ligation for miRNAcloning
GOLD ssDNA, CircLigase II
CTI021K 1,000 U
CTI025K 5,000 U
Storage
-20ºC
Description
This thermostable ligase catalyses intramolecular ligation (i.e., circularisation) of ssDNA templates >30 nt having a 5′ phosphate and a 3′ hydroxyl group. It ligates the ends of ssDNA in the absence of a complementary sequence. Standard reaction conditions produce no detectable single-stranded DNA concatamers or concatameric DNA circles. Due to the high degree of adenylation, a single CircLigase II enzyme can ligate only a single molecule of nonadenylated DNA, and the reaction stops in the absence of ATP. Therefore, a 1:1 stoichiometric amount of ligase:substrate is required to drive the ligation reaction to completion.
Application
  • Production of ssDNA templates for rolling-circle replication or rolling-circle transcription experiments
  • Production of ssDNA templates for RNA polymerase and RNA polymerase inhibitor assays
GOLD ssDNA, CircLigase
CTD111K 100 U/μL 1,000 U
CTD115K 100 U/μL 5,000 U
Storage
-20ºC
Description
This thermostable, ATP-dependent ligase catalyses intramolecular ligation (i.e., circularisation) of ssDNA templates >30 nt having a 5′ phosphate and a 3′ hydroxyl group. It ligates the ends of ssDNA in the absence of a complementary sequence. Standard reaction conditions produce no detectable singlestranded DNAconcatamers or concatameric DNA circles. Due to the low degree of adenylation, CircLigase enzyme has high turnover; it can reversibly and repeatedly act on multiple preadenylated DNAmolecules under nonstoichiometric reaction conditions.
Application
  • Production of ssDNA templates for rolling-circle replication or rollingcircle transcription experiments
  • Production of ssDNA templates for RNA polymerase and RNA polymerase inhibitor assays
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