GOLD Terminator 5'Phosphate-Dependent Ex

GOLD Terminator 5'Phosphate-Dependent Ex
CTE1020 1 U/μL 40 U
Storage
-20ºC
Description
GOLD Terminator 5’′Phosphate-Dependent Exo is a 5′→ 3′, processive exonuclease that degrades RNAs with a 5′ monophosphate. It does not degrade RNAs with a 5′ triphosphate, 5′-cap structure (such as found on most eukaryotic mRNAs), or a 5′ OH. It will also digest DNAwith a 5′ monophosphate. The enzyme is not inhibited by proteinaceous RNase inhibitors.
Application
  • Characterising the 5′ termini of RNAtranscripts
  • Preparing mRNA-enriched samples from eukaryotic or prokaryotic total RNA preparations in 1 hour without the use of oligo(dT), resins, or magnetic beads
GOLD Cleave End
CTG850K 200 U/μL 50,000 U
Storage
-20ºC
Description
This endonuclease digests all forms of DNA and RNA including single-stranded and double-stranded linear, circular, and supercoiled. OmniCleave Endonuclease has the same substrate specificity, and yields the same products as Benzonase, an enzyme derived from Serratia marcescens.
Application
  • Removal of nucleic acids from cell lysates (reduction of viscosity) for improved handling and yield of protein preparations
  • Removal of trace contamination by nucleic acids in protein preparations
  • Removal of host DNAfrom phage preparation
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