ProPrime Taq DNA Polymerase

ProPrime Taq DNA Polymerase
dNTPs-free CT1P401-01 250 units
CT1P401-02 500 units
CT1P401-03 6x500 units
dNTPs(2.5 mM) CT1P401-11 250 units
CT1P401-12 500 units
CT1P401-13 6x500 units
Concentration
2.5 units/μl
Contents
  • ProPrime Taq DNA Polymerase
  • 10×ProPrime Taq Buffer (500 mM Tris-HCl (pH 9.0); 200 mM (NH4 )2 SO4; 20 mM MgSO4; 10% glycerol etc.)
GC Enhancer
  • 6×DNA Loading Buffer
Storage
-20º C
Description
ProPrime Taq DNA Polymerase is a hot Prime Taq DNA polymerase containing Taq DNA polymerase and three kinds of monoclonal antibodies, effectively blocking DNA polymerase activity and preventing non-specific amplification at low temperature. As the denaturation step progresses, the antibodies are inactivated and Taq DNA Polymerase is activated, which can effectively enhance the amplification efficiency and improve the amplification sensitivity and specificity. The amplified product has an “A” base at the 3′ end and can be directly cloned into the pQUIK-T vectors.
  • Compared with MetaPrime Taq DNA Polymerase and MetaPrime TopTaq DNA Polymerase, ProPrime Taq DNA Polymerase has higher amplification efficiency, specificity and sensitivity.
  • Prepared at room temperature, reduced nonspecific and primer dimer formation.
  • Different from chemical modification, long denaturing step is not needed.
  • The reaction solution is specifically designed for molecular diagnosis, and the amplification length is no more than 1 kb.
Applications
  • Low copy number templates
  • Complex templates
  • GC/AT rich templates
  • Real-time Quantitative PCR (qPCR)
  • Multiplex PCR
  • Single nucleotide polymorphisms ( SNPs)
Unit Definition
One unit of ProPrime Taq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
Thermal cycling condions
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