GOLD DNA Polymerase

Iso GOLD Bst DNA Polymerase
CTC0027-1 8,000 U/mL 2,000 U
CTC0027-2 8,000 U/mL 10,000 U
CTC0028-3 50,000 U/mL 10,000 U
Storage
-20ºC
Description
Iso GOLD Bst DNA Polymerase, is a recombinant form of the 67 kDa Bacillus stearothermophilus DNA Polymerase protein (large fragment). The enzyme has 5′→3′ polymerase activity and strand-displacement activity, but it lacks 3′→5′ exonuclease activity. It also has reverse transcription activity.
Applications
  • Strand-displacement amplification
  • DNAsequencing through high GC regions
  • Rapid sequencing from nanogram amounts of DNAtemplate
NxGn GOLD phi29 DNA Polymerase
CTC0221-1 10,000 U/mL 2,000 U
CTC0221-1 10,000 U/mL 10,000 U
Storage
-20ºC
Description
NxGen GOLD phi29 DNA Polymerase (φ29 DNA Polymerase) is a highly processive enzyme with exceptional strand-displacement activity. The enzyme also contains a 3′→5′ exonuclease activity that enables proofreading capability.
GOLD Klenow DNA Polymerase
CTA1101K 10 U/μL 1,000 U
Storage
-20ºC
Description
GOLD Klenow DNA Polymerase is a DNA-dependent DNA polymerase that lacks both the 5′→3′ and 3′→5′exonuclease activities of E. coli DNA Polymerase I, from which it is derived. This N-terminal truncation of DNA Polymerase I also has two mutations (D355Aand E357A).
Applications
  • Random-primer labeling of DNA
  • Second-strand cDNAsynthesis
  • Strand-displacement amplification
GOLDSafe Enzyme Mix
CTE1100 100 U
CTE101K 1,000 U
Storage
-20ºC
Description
GOLDSafe Enzyme Mix is a unique blend of thermostable DNA polymerase and a 3′→5′ proofreading exonuclease that is capable of amplifying most difficult templates. The FailSafe enzyme provides 3-fold higher fidelity than Taq DNA polymerase, with the ability to amplify PCR products up to 20 kb. The error rate of the FailSafe Enzyme Mix is approximately 1 in 27,000–30,000.
Applications
  • PCR and multiplex PCR
  • PCR of difficult templates
  • High-sensitivity PCR
  • PCR amplification of any sequence up to 20 kb
GOLD Taq DNA Polymerase
CTH2500 5 U/μL 500 U
CTH201K 5 U/μL 1,000 U
CTH205K 5 U/μL 10,000 U
Storage
-20ºC
Description
Derived from Thermus aquaticus, this enzyme has optimal activity at temperatures above 70 °C. It has an intrinsic 5′ →3′ s t r u c t u r e – d e p e n d e n t e x o n u c l e a s e a c t i v i t y b u t l a c k s 3 ′ → 5′ proofreading exonuclease activity. The enzyme is provided with the MasterAmp PCR Enhancer, which increases the probability of obtaining the desired amplification product and the reproducibility of PCR. It improves the consistency of PCR product yields in multiplex PCR.
Applications
  • PCR and multiplex PCR amplification of DNAtemplates
GOLD Tth DNA Polymerase
CTG2250 5 U/μL 250 U
Storage
-20ºC
Description
This recombinant enzyme from Thermus thermophilus has DNA polymerase activities up to ~95 °C, as well as reversetranscriptase activity. High reaction temperatures can reduce nonspecific priming and template secondary structure. It is provided with MasterAmp PCR Enhancer.
Applications
  • PCR amplification of DNA
  • Improved PCR of DNA templates having a high degree of secondary structure
  • One-step RT-PCR of RNA
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