Dnase GOLD

DNase GOLD
CT0G15K 1 U/μL 5,000 MBU
Storage
-20ºC
Description
DNase GOLD digests dsDNA and ssDNA to mononucleotides more effectively than the commonly used bovine pancreatic DNase I. Following treatment with Baseline-ZERO DNase, even the small DNA oligonucleotides that remain after treatment with DNase I are undetectable. The enzyme provides a true zero baseline for RNART-PCR or microarray gene expression experiments.
  • Removal of genomic DNA from RNA before RT-PCR, or preparation of target RNA or cDNA for microarray analysis, especially for exon arrays or full-coverage expression analysis
  • Removal of small DNA oligonucleotides (e.g., random primers)
DNase I GOLD (RNase-Free)
CTI9905K 1 U/μL 5,000 MBU
CTI9910K 1 U/μL 10,000 MBU
Storage
-20ºC
Description
DNase I GOLD (RNase-Free) (bovine pancreas) is an endonuclease useful in removing DNA that might interfere with the characterisation, manipulation, or use of RNA, or for any application requiring highly purified DNase I, such as nick translation. This enzyme efficiently hydrolyses dsDNA and ssDNA to a mixture of short oligonucleotides and mononucleotides.
  • Elimination of template DNA following in vitro synthesis of RNAwith T7 phage RNApolymerase
  • Labeling of DNA by nick translation, in combination with Klenow or other DNApolymerases
  • Treatment of RNAbefore RT-PCR
  • Characterisation of DNA-protein interactions by DNase I footprinting
GOLD Exo I
CTD0520K 20 U/μL 20,000 U
Storage
-20ºC
Description
GOLD Exo I digests ssDNA in a 3′ 5′ direction but does not digest dsDNA. Although it requires the presence of magnesium and a free 3′-OH terminus for activity, it is active under a wide variety of buffer conditions and can be added directly into most reaction mixes
  • Removal of residual ssDNA, including oligos, from reaction mixes
  • Removal of ssDNAfrom nucleic acid mixtures
GOLD Exo III
CTD425K 200 U/μL 25,000 U
Storage
-20ºC
Description
GOLD Exo III digests duplex DNA in a 3′→ 5′ direction from a nick, a blunt end, or a 3′ recessed end, producing stretches of ssDNA on the opposite strand.
  • Production of intermediates for site-directed mutagenesis
  • Production of strand-specific radiolabeled probes
GOLD Exo VII
CTE10250 10 U/μL 250 MBU
Storage
-20ºC
Description
GOLD Exo VII has high enzymatic specificity for ssDNA and exhibits both 5′ 3′ and 3′ 5′ exonuclease activities. It is useful for rapid removal of single-stranded oligonucleotide primers from a completed amplification reaction when different primers are required for subsequent PCR. Exonuclease VII digestion of ssDNA occurs in the absence of magnesium.
  • Removal of single-stranded oligonucleotide primers after PCR
  • Minimising the effect of primers left over from previous PCRs
GOLD-SAFE ATP-Dependent DNase
CTC101K 10 U/μL 1000 U
CTC110K 10 U/μL 10,000 U
Storage
-20ºC
Description
GOLD-SAFE ATP-Dependent DNase selectively removes contaminating bacterial chromosomal DNA from cosmid, BAC, fosmid, and plasmid preparations. The enzyme will processively degrade linear DNA from the ends; closed circular DNA (e.g., a plasmid) does not have free ends and is therefore not degraded. These properties make Plasmid-Safe ATP-Dependent DNase ideal for BAC and fosmid purification protocols, such as for shotgun sequencing, and other applications where high-purity DNA is necessary
  • Removal of contaminating bacterial chromosomal DNA in large-scale plasmid, cosmid, fosmid, and BAC vector or clone preparations
GOLD Rec J Exo
CTD11250 10 U/μL 250 U
Storage
-20ºC
Description
GOLD Rec J Exo, derived from E. coli, catalyses removal of deoxyribonucleoside monophosphates from ssDNA in a 5′→3′ direction. Its activity is dependent on Mg2+. Rec J Exonuclease can be heat-inactivated by incubation at 65 °C for 20 minutes.
  • Removal of primers from completed PCRs
  • Degradation of single-stranded linear DNA in dsDNA and plasmid preparations
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