CARBUnnMag mRNA Kit
Storage
2-8° C
CARBUnnMag mRNA Kit
| CB5C511-01 | 24 rxns |
| CB5C511-02 | 96 rxns |
Description
CARBUnnMag mRNA Kit uses oligo(dT)-conjugated magnetic beads to
specifically bind to poly(A) tailed mRNA. It is suitable for isolating
mRNA from purified highly intact total RNA (0.1-10 μg, RIN value≥8).
The isolated mRNAcan be used in RT-PCR, qRT-PCR, next generation
sequencing, or other applications. This kit is compatible with
magnetic-rod high-throughput nucleic acid extractor.
mRNA Capture Effect
After using CARBUnnMag mRNA Kit to capture mRNA of total RNA from human Hela
cells, zebra fish, tobacco and corn, use ProPrime Green qPCR Supermix to quantify the
rRNA (18S RNA) and mRNA (GAPDH) before and after the capture, and calculate its ΔCt
(Ct before capture minus Ct after capture) to reflect the mRNA capture efficiency.
Storage
15-25° C
RNAhold
| CT5H101-01 | 100 ml |
Description
RNAhold is an aqueous, nontoxic tissue preservation solution. It can
inactivate RNase and keeps RNAintact by permeating cells and tissues.
Cells and tissues can be stored at this solution for one week at room
temperature without RNA degradation. It can be used for RNA
preservation from bacteria, cells and most fresh animal tissues.
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Immediate RNase inactivation.
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Sample can be stored at room temperature for 1 week, 2-8°C for 1 month, -20°C or -80°C for long term storage.
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Ideal for field sample collection.
Storage
at room temperature
RNase-free Water
| CT7I201-01 | 25 ml |
Description
RNase-free Water is prepared from deionized water incubated with
0.01% DEPC, then autoclaved to remove residual DEPC. It is suitable for
RNA-related molecular biology applications.
Storage
4° C
Others
-20° C
2×RNA Loading Buffer
| CT7H201-01 | 1 ml |
Description
This product is used as a loading buffer in RNA electrophoresis. It is
suitable for denatured or native agarose gel and polyacrylamide gel
electrophoresis. Prior to loading, add equal volume of the Loading
Buffer to the RNAsample (or RNAmarker), heat at 70°C for 10 minutes,
immediately chill on ice, and load on the gel.