CARBUnn Meta Zol Plant
Storage
15-25° C
Procedures
CARBUnn Meta Zol Plant
CB5T121-01 | 100 ml |
Description
CARBUnn Meta Zol Plant is a ready-to-use reagent for the isolation of
total RNA from polysaccharide-rich and/or polyphenol plant tissues,
such as champignon, banana fruit, mango fruit, potato, carrot,
sansevieria. It uses a modified CTAB method to lyse samples and
phenol/chloroform to remove proteins and other impurities. It is also
suitable for the isolation of total RNA from animal tissues like fat,
connective tissues.
RNA yield from different samples
Material | Amount | RNA yield |
---|---|---|
Papaya | 100 mg | ~7 μg |
Banana | 100 mg | ~8.5 μg |
Apple | 100 mg | ~4 μg |
Chinese yam | 100 mg | ~9 μg |
Pear | 100 mg | ~1.5 μg |
Chlorophytum comosum leaves | 100 mg | ~7.5 μg |
Potato tuber | 100 mg | ~7 μg |
Pine needle | 100 mg | ~2.5 μg |
Storage
-20° C
Others
15-25° C
CARBUnnEasy RNA Kit
CB5R101-01 | 50 rxns |
Description
CARBUnnEasy RNA Kit provides a simple and fast column based method
to isolate total RNA from animal cells, animal tissues, bacteria and
yeast. Cells and tissues are enzymatically lysed. DNA is digested with
DNase I. RNA is bound to the silica membrane. After washing, high
quality RNA is eluted from the column. RNA is free of protein
contamination, and is suitable for RT-PCR, qRT-PCR and Northern Blot.
Sample Requirement
Material | Amount | volume of BB4/β-ME |
---|---|---|
Animal cell | ≤5×106 | 0.3-0.6 ml |
Animal tissue | ≤20 mg | 0.3-0.6 ml |
Bacterial cell | 1≤1×109 | 0.35 ml |