CARBUnn Meta Zol
Storage
2-8° C
Procedures
CARBUnn Meta Zol
| CB5T101-01 | 100 ml |
Description
CARBUnn Meta Zol is a ready-to-use reagent for the isolation of total
RNA from cells and tissues. CARBUnn Meta Zol combines phenol and
guanidine thiocyanate in a mono-phase solution to inhibit RNase. After
lysis and centrifugation, RNA remains in the aqueous phase and others
in the interphase or organic phase. RNA is precipitated by addition of
isopropanol.
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Isolate RNA from a variety of species: animal, plant, yeast, bacteria and viruses.
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The whole procedure can be completed in one hour.
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Simultaneous isolation of RNA, DNA and protein from the same sample.
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Pink solution for easy visualizing different phases.
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Unique dissolving solution for long-term RNAstorage.
Storage
2-8° C
Procedures
CARBUnn Meta Zol Up
| CB5T111-01 | 100 ml |
Description
CARBUnn Meta Zol Up is a ready-to-use reagent for the isolation of
total RNA from cells and tissues. Unique lysis buffer is used to disrupt
cells. After centrifugation, the solution is separated into an upper
colorless aqueous phase containing RNA and a lower pink organic
phase. RNA is precipitated and recovered with isopropanol. Proteins
can be recovered from organic phase with isopropanol. Compared with
other total RNA extraction reagents, CARBUnn Meta Zol Up provides a
powerful lysis buffer to extract RNAfrom a variety of species.
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Suitable for isolating RNA from a variety of species including animal, plant and bacteria.
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Superior lysis capability and higher RNAyield.
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The whole procedure can be completed in one hour.
-
Pink solution for easy visualizing different phases.
-
Unique dissolving solution for long-term RNAstorage.
RNA yield from different samples
| Material | Amount | RNA yield |
|---|---|---|
| Tobacco | 100 mg | ~10 μg |
| Human blood | 200 μl | ~2 μg |
| HeLa cell | 2×106 cells | ~10 μg |
| Mouse liver | 100 mg | ~16 μg |
| Rat liver | 100 mg | ~28 μg |