CARBUnn Zol - Blood
Storage
15-25° C
Procedures
CARBUnn Zol - Blood
| CB5E131-01 | for 50 ml blood |
| CB5E131-02 | for 200 ml blood |
Description
CARBUnn Zol – Blood provides an easy and fast method to isolate high
quality genomic DNA from 0.1-20 ml of fresh or frozen blood. Isolated
DNA is free of contaminants and enzyme inhibitors. Red Cell Lysis
Buffer is provided to remove non-nucleated red cells and reduce
hemoglobin contamination. Genomic DNA is precipitated with
isopropanol.
-
High quality, free of contaminants and inhibitors.
-
Suitable for EDTA, sodium citrate and heparin-anticoagulated fresh and frozen blood.
-
No organic solvents.
-
Isolated DNA is suitable for PCR, restriction enzyme digestion and Southern Blot.
DNA yield from different samples
| Material | Amount | Yield |
|---|---|---|
| Human whole blood | 400 μl | ~30 μg |
| Mouse whole blood | 400 μl | ~20 μg |
Storage
15-25° C
Procedures
CARBUnn Zol - Plant
| CB5E141-01 | 100 ml |
Description
CARBUnn Zol – Plant provides an easy and fast method to isolate high quality plant genomic DNA. Plant tissue is disrupted by grinding in liquid nitrogen. DNA is released with detergent. DNA is separated from other components by centrifugation and precipitated with isopropanol.
CARBUnn Zol – Plant is suitable to isolate DNA from plants rich in polysaccharide and polyphenol.-
Isolated DNA is suitable for PCR, restriction enzyme digestion and Southern Blot.
DNA yield from different fresh plant leaves (100 mg)
| Material | Yield |
|---|---|
| Tobacco leaves | ~20 μg |
| Wheat leaves | ~35 μg |
| Rape leaves | ~9 μg |
| Rice leaves | ~29 μg |
| Soybean leaves | ~16 μg |
| Arabidopsis leaves | ~28 μg |
| Maize leaves | ~22 μg |
| Tomato leaves | ~7 μg |