pQUIK-Blunt E1 Expression Kit
pQUIK-Blunt E1 Expression Kit
| CT3E111-01 | 10 rxns |
Storage
-20° C
Description
pQUIK-Blunt E1 Expression Vector is constructed from pET vector, it
utilizes a highly efficient, five-minute blunt cloning strategy to clone
PCR product into high-efficient expression vector. It utilizes promoter
T7lac to precise control,expresses targert gene in high efficient.The
size of control insert is 750 bp, and expressed target protein is about 27
kDa.
-
5 minutes fast ligation of Pfu-amplified PCR products.
-
Ampicillin resistance.
-
T7 promoter primer and T7 terminator primer for sequencing.
-
Bacteriophage T7lac promoter for high level expression.
-
N-terminal 6xHis tag for easy purification.
-
Meta1-T1 Phage Resistant Chemically Competent Cell, high 9 transformation efficiency (>10 cfu/μg pUC19 DNA) and fast growing.
-
E1 Expression Plasmid included as negative control.
-
Simplicity:just add the fragment.
-
High efficiency:high positive rate.
-
Promoter T7lac controls expression precisely.
-
Providing ampicillin selection marker.
Kit Contents
| Component | CT3E111-01 |
|---|---|
| pQUIK-Blunt E1 Expression Vector (15 ng/μl) | 10 μl |
| E1 Expression Plasmid (Negative Control) (15 ng/μl) | 10 μl |
| Econtrol Template (5 ng/μl) | 10 μl |
| Econtrol Forward Primer (10 μM) | 10 μl |
| Econtrol Reverse Primer (10 μM) | 10 μl |
| T7 Promoter Primer (10 μM) | 50 μl |
| T7 Terminator Primer (10 μM) | 50 μl |
| Meta1-T1 Phage Resistant Chemically Competent Cell | 5×100 μl |
pQUIK-Blunt E1 Prokaryotic Expression Vector Map
- T7 promoter: bases 209-225
- T7 Transcription start: base 226
- Lac operator(lacO): bases 228-252
- RBS: bases 282-288
- His·Tag coding sequence: bases 309-326
- T7 terminator: bases 436-482
- Ampicillin resistance ORF: bases 907-1,767
- pBR322 origin: bases 1,922-2,541
- ROP ORF: bases 2,953-3,144
- LacI ORF: bases 4,459-5,547
Feature and application of pQuik expression vectors
| Name | Amp+ | Promoter | Sequenciang primer | Characteristics | Application |
|---|---|---|---|---|---|
| pQuik-Blunt E1 | + | T7lac | T7 Promoter Primer; | N-terminal | Prokaryotic Expression |
| T7 Terminator Primer | 6×His tag | ||||
| pQuik-Blunt E2 | + | T7lac | T7 Promoter Primer; | C-terminal | Prokaryotic Expression |
| T7 Terminator Primer | 6×His tag |
pQUIK-Blunt E2 Expression Kit
| CT3E211-01 | 10 rxns |
Storage
-20° C
Description
pQUIK-Blunt E2 Expression Vector is constructed from pET vector. It
utilizes a highly efficient, five-minute blunt cloning strategy to clone
PCR product into high-efficient expression vector. The size of control
insert is 750 bp, and expressed target protein is about 27 kDa.
-
5 minutes fast ligation of Pfu-amplified PCR products.
-
Ampicillin resistance.
-
T7 promoter primer and T7 terminator primer for sequencing.
-
Bacteriophage T7lac promoter for high level expression.
-
C-terminal 6×His tag for easy purification.
-
Meta1-T1 Phage Resistant Chemically Competent Cell, high 9 transformation efficiency (>10 cfu/μg pUC19 DNA) and fast growing.
-
E2 Expression Plasmid included as negative control.
Kit Contents
| Component | CT3E211-01 |
|---|---|
| pQUIK-Blunt E1 Expression Vector (15 ng/μl) | 10 μl |
| E1 Expression Plasmid (Negative Control) (15 ng/μl) | 10 μl |
| Econtrol Template (5 ng/μl) | 10 μl |
| Econtrol Forward Primer (10 μM) | 10 μ |
| Econtrol Reverse Primer (10 μM) | 10 μl |
| T7 Promoter Primer (10 μM) | 50 μl |
| T7 Terminator Primer (10 μM) | 50 μl |
| Meta1-T1 Phage Resistant Chemically Competent Cell | 5×100 μl |
