Bl21 (DE3) Chemically Competent Cell

Storage

-70° C

The expression of T7 RNA p o l y m e r a s e g e n e i s controlled by the lacUV5 promoter of the DE3 region of bacteriophageλ, which is integrated on the chromosome of Bl21.
Bl21 (DE3) Chemically Competent Cell
CT3D601-02 10×100 μl
CT3D601-03 20×100 μl
Features
  • Use pUC19 plasmid DNA detection Transformation efficiency: >10 cfu/μg (pUC19 DNA).
  • DE3 strains contains the λDE3 lysogen that carries the gene for T7 RNApolymerase.
  • Suitable for T7 and T7lac such as pET, pQUIK.
  • Suitable for high expression of non-toxic protein
  • Control plasmid I (Amp ) is used for detection of expression function of cell. The protein size is about 25 kDa.
Genotype
  • F ompT hsdS (rB- mB- ) gal dcm(DE3)
Storage

-70° C

Bl21 (DE3)pLysS Chemically Competent Cell
CT3D701-02 10×100 μl
CT3D701-03 20×100 μl
Characteristics
  • Use pUC19 plasmid DNA detection Transformation efficiency: >10 cfu/μg (pUC19 DNA).
  • CamR .
  • Contains pLysS plasmid that expresses the T7 lysozyme gene to reduce the background of the target protein expression without disturbing IPTG functioning.
  • Suitable for non-toxic and toxic protein’s expression.
  • Control plasmid III (Amp ) is used for detection of expression function of cell. The protein size is about 25 kDa.
Genotype
  • F- ompT hsdS (rB- mB- ) gal dcm(DE3) pLysSCamr
Storage

-70° C

MetaT (DE3) Chemically Competent Cell
CT3D801-02 10×100 μl
CT3D801-03 20×100 μl
Characteristics
  • Use pUC19 plasmid DNA detection Transformation efficiency: >107 cfu/μg (pUC19 DNA).
  • CamR .
  • Complement tRNAs for 6 rare codons AUA, AGG, AGA, CUA, CCC, GGA,which is lacking in E.coli. Enhance the expression level of proteins in the prokaryotic system.
  • Control plasmid I (Amp ) is used for detection of expression function of cell. The protein size is about 25 kDa.
Genotype
  • F- ompT hsdSB (rB- mB- ) gal dcm lacY1(DE3) pRARE (argU,argW, ileX, B B r glyT, leuW, proL) (Camr )
Storage

-70° C

MetaB (DE3) Chemically Competent Cell
CT3D811-02 10×100 μl
Features
  • Use pUC19 plasmid DNA detection Transformation efficiency: >107 cfu/μg (pUC19 DNA).
  • KanR and TetR .
  • Thioredoxin reductase (trxB) and glutathione reductase (gor) mutation greatly facilitates cytoplasmic disulfide bond formation
  • Control plasmid I (Amp+ ) is used for detection of expression function of cell. The protein size is about 25 kDa.
Genotype
  • F- ompT hsdSB (rB- mB- ) gal dcm lacY1 ahpC (DE3) gor522::Tn10 trxB B B R R (Kan R,TetR )
Storage

-70° C

Bl21 Chemically Competent Cell
CT3D901-02 10×100 μl
CT3D901-03 20×100 μl
Features
  • Use pUC19 plasmid DNA detection Transformation efficiency: >107 cfu/μg (pUC19 DNA).
  • TetR .
  • Suitable for the expression of toxic proteins,suitable for the Tac promoter system, such as pGEX,pMAL.
  • Control plasmid II (Amp+) is used for detection of expression function of cell. The protein size is about 26 kDa.
Genotype
  • E. coli B F dcm ompT hsdS (r B-mB- ) gal [malB+ ]K-12 ( λ 5)
Expression Competent Cells
The chemically competent cells of Meta are produced by a special process and have a higher Transformation efficiency than the competent cells produced by traditional methods.
Selection Guide
Name Cat. No. Transformation Efficiency Application
BL 21(DE3) CD 601 107 cfu/µμg DNA High expression of non-toxic protein
BL 21(DE3) pLysS CD 701 107 cfu/μg DNA High expression of toxic protein and nontoxic protein, low background
Metaetta(DE3) CD 801 107 cfu/μg DNA Contains tRNAs corresponding to 6 rare codons, application to eukaryotic gene expression
MetaB(DE3) CD 811 107 cfu/μg DNA Conducive to the formation of the correctly folded protein with disulfide, enhance the solubility of the protein
BL 21 CD 901 107 cfu/μg DNA High expression of toxic protein
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