pQuik-Uni Seamless Cloning and Assembly Kit

pQuik-Uni Seamless Cloning and Assembly Kit
CT3U101-01 10 rxns
CT3U101-02 20 rxns
CT3U101-03 60 rxns
Storage
-20° C
Description
This kit takes advantage of proprietary recombinases mix and homologous recombination. to achieve directional recombination of PCR inserts with 15-25 bp overlapping ends into any linearized vector.
  • Rapid: 15 minutes.
  • Simple: No restriction enzyme digestion
  • High efficiency: >95% cloning efficiency.
  • Seamless: No extra sequences introduced.
Kit Contents
Component CT3U101-01 CT3U101-02 CT3U101-03
2×Assembly Mix 50 μl 100 μl 3×100 μl
Meta1-T1 Phage Resistant Chemically Competent Cell 5×100 μl 6 μl 18 μl
Linearized pUC19 Control Vector (10 ng/μl) 3 µl 6 μl 18 μl
Control Insert (1 kb, 20 ng/μl) 3 μl 10×100 μl 30×100 μl
Principle
  • Prepare linearized vector by PCR/Enzyme digestion
  • PCR amplify inserts with primers containing overlapping sequences
  • Mix vector, DNA fragments and Assembly Mix together.
  • Incubate at 50 C for 15 minutes.
  • Transformation.
pQuik-Basic Seamless Cloning and Assembly Kit
CT3U201-02 20 rxns
CT3U201-03 60 rxns
Storage
-20° C
Description
This kit takes advantage of proprietary recombinases and homologous recombination to achieve directional recombination of PCR inserts with 15-25bp overlapping ends into any linearized vector, enabling efficient and seamless assembly of 1-5 fragments.
Highlights
  • Rapid: 15 minutes.
  • Simple: No restriction enzyme digestion
  • High efficiency: >95% cloning efficiency.
  • Seamless: No extra sequences introduced.
Kit Contents
Component CT3U201-02 CT3U201-03
2×Basic Assembly Mix 100 μl 3×100 μl
Linearized pUC19 Control Vector (10 ng/μl) 6 μl 18 μl
Control Insert (1 kb，20 ng/μl) 6 μl 18 μl
Meta1-T1 Phage Resistant Chemically Competent Cell 10×100 μl 30×100 μl
Principle
  • Prepare linearized vector by PCR/Enzyme digestion
  • PCR amplify inserts with primers containing overlapping sequences
  • Mix vector, DNA fragments and Assembly Mix together.
  • Incubate at 50 C for 15 minutes.
  • Transformation.
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