pQuik-T5 Zero Cloning Kit
pQuik-T5 Zero Cloning Kit
Two antibiotic resistance genes; zero background; no need for blue/white screening
| CT3T501-01 | 20 rxns |
| CT3T501-02 | 60 rxns |
Storage
-20° C
Description
This product selects positive recombinants by expression of a lethal
gene. When the vector is not ligated to the insert, the lethal gene is
expressed to kill the cells containing the vectors, enabling zero
background. It is suitable for TAcloning.
-
Rapid: 5 minutes only
-
Easy: Only adding the insert is needed
-
Highly efficient: almost 100% high positive rate.
-
Low background: close to zero
-
No blue/white selection needed.
-
Suitable for short and large fragment cloning.
-
Kanamycin and Ampicillin resistance genes for selection.
-
M13 forward primer and M13 reverse primer for sequencing.
-
T3 promoter and T7 promoter for in vitro transcription
-
Meta1-T1 Phage Resistant Chemically Competent Cell Features, high transformation efficiency and Rapid growth, generating sufficient colonies to save time.
Kit Contents
| Component | CT3T101-01 | CT3T101-02 |
|---|---|---|
| pQuik-T5 Zero Cloning Vector (10 ng/μl) | 20 μl | 3×20μl |
| Control Template (5 ng/μl) | 5 μl | 5 μl |
| Control Primers (10 μM) | 5 μl | 5 μl |
| M13 Forward Primer (10 μM) | 50 μl | 150 μl |
| M13 Reverse Primer (10 μM) | 50 μl | 150 μl |
| Meta1-T1 Phage Resistant Chemically Competent Cell | 10×100 μl | 30×100 μl |
pQuik-Blunt Zero Cloning Kit
Two antibiotic resistance genes; zero background; no need for blue/white screening
| CT3B501-01 | 20 rxns |
| CT3B501-02 | 60 rxns |
Storage
-20° C
Description
This product selects positive recombinants by expression of a lethal
gene. When the vector is not ligated to the insert, the lethal gene is
expressed to kill the cells containing the vectors, enabling zero
background. It is suitable for blunt cloning.
-
Rapid: 5 minutes only
-
Easy: Only adding the insert is needed
-
Highly efficient: almost 100% high positive rate.
-
Low background: close to zero
-
No blue/white selection needed.
-
Suitable for short and large fragment cloning.
-
Kanamycin and Ampicillin resistance genes are available for selection convenient for selection according to experiment conditions.
-
M13 forward primer and M13 reverse primer for sequencing.
-
T3 promoter and T7 promoter for in vitro transcription
-
Meta1-T1 Phage Resistant Chemically Competent Cell Features, high transformation efficiency and Rapid growth, generating sufficient colonies to save time.
Kit Contents
| Component | CT3T101-01 | CT3T101-02 |
|---|---|---|
| pQuik-Blunt Zero Cloning Vector (10 ng/μl) | 20 μl | 3×20μl |
| Control Template (5 ng/μl) | 5 μl | 5 μl |
| Control Primers (10 μM) | 5 μl | 5 μl |
| M13 Forward Primer (10 μM) | 50 μl | 150 μl |
| M13 Reverse Primer (10 μM) | 50 μl | 150 μl |
| Meta1-T1 Phage Resistant Chemically Competent Cell | 10×100 μl | 30×100 μl |
