pQuik-T3 Cloning Kit
pQuik-T3 Cloning Kit
Double antibiotic resistance genes, quick cloning
| CT3T301-01 | 20 rxns |
| CT3T301-02 | 60 rxns |
Storage
-20° C
Description
This vector carries the LacZ gene which enables blue/white screening
on plates containing mediums with IPTG and X-gal. It is suitable for TA
cloning
-
Rapid: Only 5 minutes
-
Easy: Only adding the insert is needed
-
Highly efficient: high positive rate.
-
Convenient: two digestion sites are available, EcoR I and Not I for digestion verification.
-
Ampicillin is provided for screening
-
T7 promoter, SP6 promoter, M13 forward and M13 reverse primers for sequencing.
-
T7 promoter and SP6 promoter for in vitro transcription.
-
Meta1-T1 Phage Resistant Chemically Competent Cell features, high transformation efficiency and Rapid growth, generating sufficient colonies to save screening time.
Kit Contents
| Component | CT3T101-01 | CT3T101-02 |
|---|---|---|
| pQuik-T3 Cloning Vector (10 ng/μl) | 20 μl | 3×20μl |
| Control Template (5 ng/μl) | 5 μl | 5 μl |
| Control Primers (10 μM) | 5 μl | 5 μl |
| M13 Forward Primer (10 μM) | 50 μl | 150 μl |
| M13 Reverse Primer (10 μM) | 50 μl | 150 μl |
| Meta1-T1 Phage Resistant Chemically Competent Cell | 10×100 μl | 30×100 μl |
pQuik-Blunt3 Cloning Kit
Quick cloning for blunt ends
| CT3B301-01 | 20 rxns |
| CT3B301-02 | 60 rxns |
Storage
-20° C
Description
This vector carries the LacZ gene which enables blue/white screening
on plates containing mediums with IPTG and X-gal. It is suitable for
blunt cloning.
-
Rapid: 5 minutes only.
-
Easy: Only adding the insert is needed.
-
Highly efficient: high positive rate
-
Convenient: two digestion sites are available, EcoR I and Not I for digestion verification.
-
Ampicillin is provided for screening.
-
T7 promoter, SP6 promoter, M13 forward and M13 reverse primers for sequencing.
-
T7 promoter and SP6 promoter for in vitro transcription.
-
Meta1-T1 Phage Resistant Chemically Competent Cell, high transformation efficiency and Rapid growth, generating sufficient colonies to save screening time.
Kit Contents
| Component | CB101-01 | CB101-02 |
|---|---|---|
| pQuik-Blunt3 Cloning Vector (10 ng/μl) | 20 μl | 3×20μl |
| Control Template (5 ng/μl) | 5 μl | 5 μl |
| Control Primers (10 μM) | 5 μl | 5 μl |
| M13 Forward Primer (10 μM) | 50 μl | 150 μl |
| M13 Reverse Primer (10 μM) | 50 μl | 150 μl |
| Meta1-T1 Phage Resistant Chemically Competent Cell | 10×100 μl | 30×100 μl |
