pQuik-T1 Simple Cloning Kit
pQuik-T1 Simple Cloning Kit
Double antibiotic resistance genes, quick cloning
| CT3T111-01 | 20 rxns |
| CT3T111-02 | 60 rxns |
Storage
-20° C
Description
This product eliminates the multi-cloning sites of pQuik-T1 Cloning
Vector. The PCR products after cloning cannot be digested by
restriction enzymes from multiple cloning sites on pQuik-T1 Cloning
Vector. This vector carries the LacZ gene which enables blue/white
screening on plates containing medium with IPTG and X-gal. It is
suitable for TAcloning.
-
Rapid: Only 5 minutes
-
Easy: Only adding the insert is needed.
-
Highly efficient: high positive rate.
-
Flexible: two antibiotics are provided for screening: kanamycin and ampicillin, convenient to select according to experiment.
-
SR primer and M13 forward primer for sequencing.
-
T7 promoter for in vitro transcription.
-
Meta1-T1 Phage Resistant Chemically Competent Cell features, high transformation efficiency and Rapid growth, generating sufficient colonies to save screening time.
Kit Contents
| Component | CT3T111-01 | CT3T111-02 |
|---|---|---|
| pQuik- T1 Cloning Vector(10 ng/μl) | 20 μl | 3×20μl |
| Control Template (5 ng/μl) | 5 μl | 5 μl |
| Control Primers (10 μM) | 5 μl | 5 μl |
| M13 Forward Primer (10 μM) | 50 μl | 150 μl |
| M13 Reverse Primer (10 μM) | 50 μl | 150 μl |
| SR Primer (10 μM) | 50 μl | 150 μl |
| Meta1-T1 Phage Resistant Chemically Competent Cell | 10×100 μl | 30×100 μl |
pQuik-Blunt Simple Cloning Kit
| CT3B111-01 | 20 rxns |
| CT3B111-02 | 60 rxns |
Storage
-20° C
Description
This product eliminates the multi-cloning sites of pQuik-T1 Cloning
Vector. The PCR products after cloning cannot be digested by
restriction enzymes from multiple cloning sites on pQuik-T1 Cloning
Vector. This vector carries the LacZ gene which enables blue/white
screening on plates containing medium with IPTG and X-gal. It is
suitable for TAcloning.
-
Rapid: Only 5 minutes
-
Easy: Only adding the insert is needed.
-
Highly efficient: high positive rate.
-
Flexible: two antibiotics are provided for screening: kanamycin and ampicillin, convenient to select according to experiment.
-
SR primer and M13 forward primer for sequencing.
-
T7 promoter for in vitro transcription.
-
Meta1-T1 Phage Resistant Chemically Competent Cell features, high transformation efficiency and Rapid growth, generating sufficient colonies to save screening time.
Kit Contents
| Component | CT3T111-01 | CT3T111-02 |
|---|---|---|
| pQuik- T1 Cloning Vector(10 ng/μl) | 20 μl | 3×20μl |
| Control Template (5 ng/μl) | 5 μl | 5 μl |
| Control Primers (10 μM) | 5 μl | 5 μl |
| M13 Forward Primer (10 μM) | 50 μl | 150 μl |
| M13 Reverse Primer (10 μM) | 50 μl | 150 μl |
| SR Primer (10 μM) | 50 μl | 150 μl |
| Meta1-T1 Phage Resistant Chemically Competent Cell | 10×100 μl | 30×100 μl |
