pQuik-T1 Cloning Kit
pQuik-T1 Cloning Kit
Double antibiotic resistance genes, quick cloning
| CT3T101-01 | 20 rxns |
| CT3T101-02 | 60 rxns |
Storage
-20° C
Description
This product contains LacZ gene which can be used for blue/white
screening on plates with IPTG and X-gal.
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Rapid: Only 5 minutes
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Simple: Ready after adding the insert
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Easy blue/white selection.
-
High Efficiency: high positive clones
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Ampicillin- and kanamycin-resistance genes for screening, can be selected based on your experiment.
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Multiple convenient restriction sites for easy subcloning.
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T7 promoter, M13 forward and M13 reverse primers for sequencing.
-
T7 promoter for in vitro transcription.
High transformation efficiency, Rapid growth of Meta1-T1 competent
cells, enabling adequate clones and saving time.
Kit Contents
| Component | CT3T101-01 | CT3T101-02 |
|---|---|---|
| pQuik- T1 Cloning Vector(10 ng/μl) | 20 μl | 3×20μl |
| Control Template (5 ng/μl) | 5 μl | 5 μl |
| Control Primers (10 μM) | 5 μl | 5 μl |
| M13 Forward Primer (10 μM) | 50 μl | 150 μl |
| M13 Reverse Primer (10 μM) | 50 μl | 150 μl |
| Meta1-T1 Phage Resistant Chemically Competent Cell | 10×100 μl | 30×100 μl |
pQUIK-Blunt Cloning Kit
Double antibiotic resistance genes, quick cloning
| CT3T101-01 | 20 rxns |
| CT3T101-02 | 60 rxns |
Storage
-20° C
Description
This vector carries the LacZ gene which enables blue/white screening
on plates containing medium with IPTG and X-gal. It is suitable for
blunt cloning.
-
Rapid: 5 minutes only.
-
Easy blue/white selection.
-
Easy: Only adding the insert is needed.
-
Highly efficient: high positive rate
-
Flexible: two antibiotics are provided for screening: kanamycin and ampicillin, convenient to select according to experiment.
-
T7 promoter, M13 forward and M13 reverse primers for sequencing.
-
T7 promoter for in vitro transcription.
High transformation efficiency, Rapid growth of Meta1-T1 competent
cells, enabling adequate clones and saving time.
Kit Contents
| Component | CB101-01 | CB101-02 |
|---|---|---|
| pQuik-Blunt Cloning Vector (10 ng/μl) | 20 μl | 3×20μl |
| Control Template (5 ng/μl) | 5 μl | 5 μl |
| Control Primers (10 μM) | 5 μl | 5 μl |
| M13 Forward Primer (10 μM) | 50 μl | 150 μl |
| M13 Reverse Primer (10 μM) | 50 μl | 150 μl |
| Meta1-T1 Phage Resistant Chemically Competent Cell | 10×100 μl | 30×100 μl |
