IPTG
IPTG
| CT7F101-01 | 1 ml |
Concentration
500 mM
Storage
-20° C
Description
Isopropylthio-β-galactoside (IPTG) is an effective inducer, which cannot be
metabolized by bacteria and therefore remains constant. It functions as an
inducer of galactosidase activity. Based on the principle of α-complementation of
the β-galactosidase gene, recombinants generated from strains containing
lacZΔM15 mutation and plasmids containing the α-complementation cloning MCS,
can be identified by white coloration of cells grown in plates containing an IPTG
and X-gal.It is also used as an inducer of protein expression in T7, T7lac or tac
promoter -regulated expression vectors.
X-Gal
| CT7F201-01 | 1 ml |
Concentration
20 mg/ml
Storage
-20° C
Description
X-gal is a colorless substrate of β-galactosidase which hydrolyzes X-gal to yield an
intense blue precipitate. It is commonly used with IPTG (CT7F101) to detect βgalactosidase activity in cloning applications for blue/white screening to
differentiate recombinants from non-recombinants.
Ampicillin
| CT7G101-01 | 1 ml |
Concentration
100 mg/ml
Storage
-20° C
Description
Ampicillin is a derivative of penicillin, which interferes with bacterial cell-wall
synthesis to kill the growing cells. Its resistance relies mainly on the specific
expression of periphery β-lactamases by the resistance gene (bla). This enzyme
can hydrolyze the β-lactam ring and thus inactivate the antibiotics.
Working concentration: 20 μg/ml for stringent plasmids; 60 μg/ml for relaxed
plasmids.
Kanamycin
| CT7G201-01 | 1 ml |
Concentration
50 mg/ml
Storage
-20° C
Description
Kanamycin is an antibiotic which binds the 30s ribosomal subunit, causing
misreading of the mRNAsequence and inhibition of protein synthesis. Its resistance
is mainly conferred by specific expression of aminoglycoside phosphotransferase
from the kanamycin-resistant gene (Kan), which modifies the antibiotic and
inhibits the binding with ribosome.Working concentration: 10 μg/ml for stringent
plasmids; 50 μg/ml for relaxed plasmids.
Chloramphenicol
| CT7G301-01 | 1 ml |
Concentration
34 mg/ml
Storage
-20° C
Description
Chloramphenicol is a broad-spectrum antibiotic which inhibits bacterial protein
synthesis by binding to the 50S ribosomal subunit. The binding interferes with
peptidyl transferase activity, thereby preventing transfer of amino acids to the
growing peptide chains and blocks peptide bond formation. As a result bacterial
protein synthesis is blocked and impedes bacterial cell proliferation. Its resistance
is mainly due to the production of a specific inactivating chloramphenicol
acetyltransferase (CAT) which inactivates the antibiotic by acetylation.
Rapid Mutagenesis System
| CT6M111-01 | 10 rxns |
| CT6M111-01 | 20 rxns |
Storage
-70° C
Description
This kit uses 2×TransStart RapidPfu PCR SuperMix to amplify the
methylated template plasmid, with mutation sites in partial overlaps
designed in primers. The amplified product is digested with DMT restriction
enzyme to remove the methylated template plasmid before
transformation in a competent cell which can also digest methylated
template plasmid. It features high mutation efficiency and simple
operation.
Kit Contents
| Component | CT6M111-01 | CT6M111-02 |
|---|---|---|
| 2×TransStartRapidPfu FlyPCR SuperMix | 250 μl | 500 μl |
| DMT Enzyme (10 units/μl) | 10 μl | 20 μl |
| DMT Chemically Competent Cell | 10×50 μl | 20×50 μl |
| Nuclease-free Water | 1 ml | 1 ml |
| Scontrol Plasmid (5 ng/μl) | 10 μl | 20 μl |
| Scontrol Primers (10 μM ) | 10 μl | 20 μl |
Rapid MultiSite Mutagenesis System
| CT6M201-01 | 10 rxns |
Concentration
50 mg/ml
Storage
-20° C
Description
Rapid MultiSite Mutagenesis System is used for generating mutated PCR
fragments by introducing mutation sites on overlapping regions. High
fidelity TransStart RapidPfu Fly PCR SuperMix is included
for amplification. This kit uses proprietary assembly mix and homologous
recombination to seamlessly assemble up to six mutagenesis fragments.
-
Rapid: Fragments amplified with Rapid & high-fidelity 2×TransStart RapidPfu Fly PCR SuperMix; 15 minutes for recombination reaction.
-
Flexible: Enabling single or multi-site, continuous or discontinuous mutagenesis; at any site.
-
Efficient: >90% mutagenesis efficiency.
Kit Contents
| Component | CT6M111-01 |
|---|---|
| 2×TransStart RapidPfuPCR SuperMix | 1 ml |
| DMT Enzyme (10 units/μl) | 30 μl |
| 2×Assembly Mix | 50 μl |
| DMT Chemically Competent Cell | 10×50 μl |
| Nuclease-free Water | 1 ml |
Applicable range
plasmid ≤ 20kb