GOLD BAC Cloning Kits
GOLD BAC Cloning Kits
(pSMART BAC and pEZ BAC)
Storage
-20° C
Description
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Efficiently clone any size insert up to 200kb.
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Create libraries from A/T- or G/C-rich genomes.
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Clone gene clusters or operons.
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High insert stability & Inducible copy number
GLOD BAC cloning kits offer unprecedented accuracy, reliability,
and efficiency in BAC cloning and library construction. The
pSMART BAC or pEZ BAC vectors incorporate the CloneSmart
transcription-free technology to minimize cloning bias.
| Products | Cat. No. |
|---|---|
| GOLD pEZ BAC Blunt Cloning Kit | CTD2009, CTD2016 |
| GOLD pEZ BAC BamHI Cloning Kit | CTD2007, CTD2012 |
| GOLD BAC Cloning Kit, pSMART BAC BamHI | CTD2030 |
| GOLD BAC Cloning Kit, pSMART BAC EcoRI | CTD2031 |
| GOLD BAC Cloning Kit, pSMART BAC HindIII | CTD2032 |
| GOLD Fosmid Cloning Kit | CTD2027 |
Figure : pSMART BAC and pEZ BAC. ori2, repE, IncC – single-copy origin of
replication; oriV – inducible origin of replication; par A,B,C – partition genes;
Cmr – chloramphenicol-resistance gene; cosN – lambda packaging signal; T –
CloneSmart transcription terminators; sacB -sucrase gene lacZ – alpha
peptide of the beta galactosidase gene. Approximate positions of sequencing
primers and transcription terminators are indicated.
GOLD DNA Ligation and Transformation Kits
Storage
-20° C
Description
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Reagents for ligation and transformation.
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Fast, simple procedure
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10X more recombinants from every reaction!
Note
For further details please contact us at [email protected]
GOLD Rapid Ligation Kit
Storage
-20° C
Description
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Ligation reaction is transformation-ready in minutes
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No DNAcleanup step required
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Highly reliable and reproducible results
Note
For further details please contact us at [email protected]
GOLD Bst Adapter Fill-In Kit
| CTD0031-1 | 10 rxns |
Storage
-20° C
Description
Next Generation (NexGen) Sequencing, PCR amplification of damaged
DNA, and other applications in molecular biology result in nicks or gaps
in DNA. Ligation of non-phosphorylated adapters (or linkers) in NexGen
sequencing leaves a single-strand nick that would prevent subsequent
amplification of the internal sequence. The Bst Adapter Fill-In Kit
combines the strand displacement polymerase from Bacillus
stearothermophilus (Bst) with an optimized buffer containing
nucleotides to eliminate nicks and gaps in sample DNA.
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Close gaps remaining after linker or adapter ligation. Repair nicked DNA.
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10X more recombinants from every reaction!
Note
For further details please contact us at [email protected]